Transfection Method Rat Hepatocytes?

Mike Roper roper-m at titania.UCHSC.edu
Fri Sep 22 13:19:31 EST 1995


In article <43r311$28e at amcnix.amc.uva.nl>, "Martijn Groenink (expegen1)" <groenink> says:
>
>I am looking for a good method to transfect primary rat hepatocytes.
>
>M.Groenink at amc.uva.nl
>

Try letting the culture recuperate 24-48 h, then use lipofectin...or,
even better, try DOTMA (Syntex).  It's not commercially available, but
you may be able to get it as a gift.

good luck

Mike Roper
U Colo Hlth Sci Ctr



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