HELPÙ EtBr disposal
shelley at DARWIN.SFBR.ORG
Fri Sep 22 13:10:35 EST 1995
I have successfully used a method in the past outlined in:
G. Lunn and E.B. Sansone. 1989. Decontamination of Ethidium Bromide
Spills. APPL. IND. HYG. 1989. vol 4; no. 9:234-237.
It involves using a solution of sodium nitrite and hypophosphorous acid.
it to decontaminate surfaces, but I have also used it to decontaminate
solutions. It has two disadvantages. You have to make fresh solution
each time you use it, and it gives off nitrogen dioxide, a toxic gas, and
so should be performed under a hood.
We now routinely use a product by Schleicher & Schuell called
'Extractor' EtBr waste reduction system. It extracts EtBr from your
solution when you pass it over a 'filter'. It is very simple to use,
and, for us, has been very economical.
Hope this helps.
> Hi everybody,
> Does anybody know a safe way for disposal of EtBr? In the lab I'm working
> in they simply dilute it in water, but if there's a better way, I'd like to
> know about it. The problem is that we have no easy access to places in which
> they can treat it, so we have to manage the disposal on our own. I'd welcome
> any kind of suggestion, thanks.
> Adela Torres
> (Valencia, Spain)
More information about the Methods