UDG cloning

Dufton Christopher dufton
Fri Sep 22 15:39:30 EST 1995


I am about to start my first attempt at UDG cloning and have a tech question.
Does the 3' end of your primers need to end in U or not for proper annealing to
occur after UDG digestion?  If not then how far can the last 3'terminal U be
from the end of the primer?  Or is there in vivo clipping of the extra
nucleotides?

please post 
thanks-Chris




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