freeze-drier for HPLC fractions

Peter Barling p.barling at auckland.ac.nz
Sun Sep 24 01:22:31 EST 1995


In article <43mfu7$qu6 at mserv1.dl.ac.uk>, ds4 at st-andrews.ac.uk says...
>
>
>Dear netters,
>
>We are doing a lot of reverse phase HPLC separation of peptides
>with ACN gradients in 0.1% TFA. We need to freeze-dry our HPLC
>fractions and are currently thinking about obtaining our own
>freeze-drying system. Several people have told me that neither ACN
>nor TFA are particularly good for the pump. So, does anyone know
>about what type of (cheap!) pump may be best suited for the job?
>Any experiences?
>
>Thanks for your advise!
>
>
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>Denni Schnapp               
>Gatty Marine Laboratory                         Fax: (0)1334-463443
>University of St. Andrews                       e-mail: ds4 at st-and.ac.uk
>St. Andrews Fife KY16 8LB                       
>Scotland                        
http://www.st-and.ac.uk/~www_sa/personal/ds4/
>                     
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In my experience, it is the temperature of the cold-trap that is most 
imortant, because this governs the vapour pressure of the chamber.  You 
need at least -100oC to sucessfully freeze-dry both aqueous samples and 
those with MeCN at the same time.




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