duplicate ORI construct?

h9290184 at hkucc.hku.hk h9290184 at hkucc.hku.hk
Sun Sep 24 17:14:59 EST 1995

Hi netters,
A friend of mine was asked to make the following construct: he is to ligate the
linearized pCATbasic(from Promega) into another plasmid that contain a promoter
that they want to study. In short the resulting construct would be a plasmid
that consist of two "vectors" ligated together with the duplication of the ORI
and the AMP gene and etc. What is the likelyhood of success for such a
construct? I think they need the whole pCAT first for the CAT reporter and the
rest of the plasmid as a stuffer fragment to study the expression of the
neighbouring gene, something to do with spacial proximity and such.
Any comments appreciated either posted here or at this address.

K. Chan

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