duplicate ORI construct?

h9290184 at hkucc.hku.hk h9290184 at hkucc.hku.hk
Sun Sep 24 17:14:59 EST 1995


Hi netters,
A friend of mine was asked to make the following construct: he is to ligate the
linearized pCATbasic(from Promega) into another plasmid that contain a promoter
that they want to study. In short the resulting construct would be a plasmid
that consist of two "vectors" ligated together with the duplication of the ORI
and the AMP gene and etc. What is the likelyhood of success for such a
construct? I think they need the whole pCAT first for the CAT reporter and the
rest of the plasmid as a stuffer fragment to study the expression of the
neighbouring gene, something to do with spacial proximity and such.
Any comments appreciated either posted here or at this address.
Thanks,

K. Chan
IMB/HKU




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