IS IT OK TO LIGATE AT RT OVERNIGHT?
popa0206 at PO-Box.McGill.CA
Mon Sep 25 16:15:30 EST 1995
> mkennedy at chmeds.ac.nz (Martin Kennedy) writes:
> In article <43lfht$ilk at nntp.ucs.ubc.ca>, bina at unixg.ubc.ca (rew) writes:
> > 1. I DO NOT HAVE ACCESS TO A 15C INCUBATOR AND WAS WONDERING IF
ANYONE CAN TELL
> > ME IF THER IS A PROBLEM WITH DOING LIGATIONS AT RT OVERNIGHT?
> Try checking the temperature of the butter conditioner in your
> fridge. Ours runs at 16deg, often they are at 14 deg. Ligase
> works fine at RT, but if you are doing it overnight maybe you
> ought to just do it at 4 deg - that works fine.
I always do my ligations at 16 degrees o/n I have heard of lots of different protocols at just about
every temp. even 65 degrees for 5 minutes before then ice then add ligase and leave o/n at 4
Just about everything works if you add enough enzyme : )
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