Killing Taq

jim hartley jhartley at access2.digex.net
Mon Sep 25 11:47:51 EST 1995


Has anyone tried inactivating Taq polymerase (so restriction enzyme ends 
are not blunted) by zapping the dNTPs with alkaline phosphatase? Protocol 
would be easy: add CIAP, incubate, kill with 94 C incubation, then 
ethanol precipitate and cut with RE.  Seems like it should work.



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