mmzsm at mmn2.med.nottingham.ac.uk
Mon Sep 25 09:27:47 EST 1995
Hope someone out there will be able to give me some advice with
regards polyadenylation sequences.
1) I'm attempting to modify a vector and in the process have to
remove the polyadenylation sequence - ideally I'd like to put a
multiple cloning site before the polyadenylation sequence but will
this affect gene expression ?
2) One of the genes I'm interested in is CAT - does CAT require a
polyadenylation signal or is it stable enough without one ?
3) Is there a minimum length required between the polyA sequence and
the gene for the polyA to be effective ?
4) Is there a good review on polyA sequence structure and function ?
I did a lit. search but found very little info.
Thanks for all your help.
CRC Department of Clinical Oncology
University of Nottingham
e-mail: stewart.martin at nott.ac.uk
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