Help explain my DNA bands smearing
rafael at howard.genetics.utah.edu
Mon Sep 25 16:27:15 EST 1995
On 25 Sep 1995, jene wrote:
> Hello, recently I did a gel electrophoresis on genomic DNA isolated from
> Arabidopsis. The samples were restricted with MluI, EcoRI and SAU3A.
> I expected to see distinct bands of DNA but ended up with smears in all
> my lanes.
Why did you expect that? I would expect a big smear.
A smear is the result of many bands. With that digestion, you get many
different bands, and the range of size is almost continous: there are
many bands, each one very close to the next one. That because you don't
see any band.
Most of the enzymes give a smear when you digest chromosomal DNA with
them. Except, of course, those that cut very rarely in your genome.
That's the theory of the pulsed filed electrophoresis.
Rafael Maldonado | La cita ha sido
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