Can you use a DNA ladder in a MOPS gel?

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Tue Sep 26 01:01:03 EST 1995



We were out of RNA ladder so I used a lambda/HindII DNA ladder today in a 
1X MOPS/0.22M formaldehyde gel.  It didn't work very well (I put the DNA 
in TE/dye and heated it to 98 degrees for a few minutes before loading.  
The RNA sampels were in a formamide/formaldehyde loading buffer.)  Any 
hints on making this work if possible?





More information about the Methods mailing list