thermostable reverse tra

Jose Carballido carballido at DNAX.ORG
Tue Sep 26 23:34:05 EST 1995


         Reply to:   RE>thermostable reverse transcriptase

Hi Michael,
Perkin Elmer has a  DNA Polymerase (rTth DNA POlymerase N808-0098) that can be
used to reverse transcribe RNA. I am currently using for one step RT In Situ
PCR ans works fine.
 

--------------------------------------
Date: 9/26/95 02:19 AM
To: Jose Carballido
From: Michael Kertesz
Where can I buy thermostable reverse transcriptase? And is it really 
thermoSTABLE (i.e. would it perhaps be possible to cycle with it?), or is it 
just less unstable to high temperatures than the usual RT's?

thanks,



Michael Kertesz

ETH-Microbiology,
ETH-Zentrum/LFV,
CH-8092 Zurich, Switzerland

tel: +41-1-632 3357
fax: +41-1-632 1148
e-mail: kertesz at micro.biol.ethz.ch


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To: methods-and-reagents at net.bio.net
From: kertesz at micro.biol.ethz.ch (Michael Kertesz)
Subject: thermostable reverse transcriptase
Date: Tue, 26 Sep 1995 09:08:00
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