Home-Made TA cloning

gerald forrest gforrest at smtplink.coh.org
Wed Sep 27 11:59:08 EST 1995

We have always been successful cloning PCR fragments into a vector 
described by Holton and Graham.  Holton, T.A. and Graham, M.W. (1991) A 
simple and efficient method for direct cloning of PCR products using 
ddt-tailed vectors. Nucleic Acids Research, 19:1156. 

Jerry Forrest
Department of Biology
Beckman Research Institute/City of Hope
Duarte, CA

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