PCR specificity?

Beth Wapelhorst weazel at seanet.com
Wed Sep 27 20:03:05 EST 1995


I always try to make pretty longish primers - 60 to 70 degrees annealing, 
and this will improve specificity even though the annealing is higher. 
Also, I've noticed a difference in polymerases: Thermus aquaticus is more 
specific than thermus thermophilus. Don't ask me why, I have no idea, it 
just is. 
beth
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