purification of 100 bp fragments
Thu Sep 28 10:09:15 EST 1995
In article <446cal$tlt at taco.cc.ncsu.edu>, jwlafoll at unity.ncsu.edu (James
Warren Lafollette) wrote:
> I have been trying to find an efficient method to purify small fragments,
> between 50 - 120 bp, from agarose gels and have not been succesful. Kits
> like Geneclean only give minimal recovery. Does anyone have a protocol
> that gives a good, 50-70%, recovery of small fragments. Thanks, Jamie
I have used Bio101s Mermaid kit for isolating 100 bp fragments from gels.
My recovery was probably around 50%, which isn't great, but the isolated
DNA cloned OK.
Hope this helps
ksnowden at bio.tamu.edu
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