purification of 100 bp fragments

bio.tamu.edu bio.tamu.edu
Thu Sep 28 10:09:15 EST 1995


In article <446cal$tlt at taco.cc.ncsu.edu>, jwlafoll at unity.ncsu.edu (James
Warren Lafollette) wrote:

> I have been trying to find an efficient method to purify small fragments, 
> between 50 - 120 bp, from agarose gels and have not been succesful.  Kits 
> like Geneclean only give minimal recovery.  Does anyone have a protocol 
> that gives a good, 50-70%, recovery of small fragments.  Thanks, Jamie
> 

Hi,

I have used Bio101s Mermaid kit for isolating 100 bp fragments from gels. 
My recovery was probably around 50%, which isn't great, but the isolated
DNA cloned OK.

Hope this helps
Kim Snowden
ksnowden at bio.tamu.edu

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