Getting rid of Taq polymerase, HELP

Anne Spurkland anspurkl at bioslave.uio.no
Thu Sep 28 03:21:56 EST 1995


Petri Kursula (pkursula at zombie.oulu.fi) wrote:
: I know I've seen it somewhere... how do you get rid of Taq before 
: restriction digestion of the PCR products? Should I just extract with 
: phenol and ethanol precipitate or what? People that have experience with 
: this , please help, otherwise I'll lose my mind :-)

: Pete

Glassmilk purification works fine, at least for PCR products above 2-300 bp.

Anne




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