Can't find the gene!
hilden at unigen.unit.no
Fri Sep 29 07:55:06 EST 1995
Hallo out there.
I am trying to clone a mouse-gene for which the human sequence is
known.I have tryed PCR-based techniques with degenerate primers for
a conserved region of the human gene. I get loads of bands, even at
high temperatures, but none of them seems to be the right one.
I have cycle sequenced them and cloned them, but this doesn't give me
additional information. Is it wise to choose degenerate primers from
conserved areas? Or should I rather pick primers for a less conserved
area to avoid the problem with other genes containing the same motives?
Resently I have done Souhtern blotting with a humane-specific probe,
but I don't get any signal even at low stringency. This despite of
the fact the the gene is very much conserved.
Anyone with ANY idea, please contakt me: I am going desperate.
My postal address:
UNIGEN-Center for molecular biology
hilde.nilsen at unigen.unit.no
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