Oligonucleotide staining

Chia Jin Ngee Genie at MAILHOST.NET
Wed Sep 27 23:52:08 EST 1995


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In an article "Juergen Sasse (Path)"  <js1 at mole.bio.cam.ac.uk> wrote: 
: Hello Netters,
: 
: Does anybody have a quick and easy staining method for oligonucleotides 
: (20-50mers) separated by PAGE?

Run the oligo into a mini PAGE and when finished, soak the gel in a soln
of 0.0002% Methylene Blue and 0.1X TBE. Find something to do for 4 hours
like growing your other stuff and reading a journal. Come back and walla!
Bands appear. Cut out the bands and do your usual elution procudure, desalt.

Chia Jin Ngee
Genie at MAILHOST.NET

Institute of Molecular and Cell Biology
National University of Singapore
10 Kent Ridge Crescent
SINGAPORE 0511

All views are mine alone. Grievances may be settled over a VF2 match.

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