(no subject)

[rey garcia]

HELP! URGENT!

In semi-quantitative RT-PCR, is it valid to use beta-actin as an 
internal standard when comparing the expression of a gene in DIFFERENT 
TISSUES? Or is it only valid when comparing the expression of a gene in 
a specific tissue at different developmental stages? 

If the answer to question 1 is "no", what internal standard is best to 
use? Please e-mail responses to the address below:

rey.garcia at stonebow.otago.ac.nz

Thanks heaps.