Methods September 1995 Archives by thread

Messages sorted by: [ subject ] [ author ] [ date ] Index

Starting: Fri Sep 1 01:16:02 EST 1995
Ending: Sat Sep 30 22:13:44 EST 1995
Messages: 1368

PCR-mutagenesis Takehiko Saito
- PCR-mutagenesis Dan Deaguiar
- PCR-mutagenesis Dan Deaguiar
  - PCR-mutagenesis Dr. Ted
Direct seq: removal of PCR primers and EXO rxn Jonathan Patrick Burke
citation software RBurnap
- citation software Kojo Mensa-Wilmot
- citation software Dan Deaguiar
  - citation software Yun-Long Du, Ph.D.
    - citation software ToxicTish
    - citation software Michael Bausher
Unreliable blue/white screening Jarkko Kortesmaa
propidium iodide staining to measure apoptosis? Ildo Nicoletti, MD
Where to go for chromosomal localization of a gene? aforus
quantitative RT-PCR Todd Miller - Pharmacology
- quantitative RT-PCR Srinivas K. Janardan, M.D.
HELP: PCR Subtractive Hybridisation/reliable method wanted. g.coulton
Antibody for His-Tag Tomas Drgon
Quantitative PCR/ HELP g.coulton
Plasmid drawing software? Tomas Drgon
DNA zol ynakao at kean.ucs.mun.ca
Plasmid drawing software? Joan Boyce
test K. Legate
primer restriction sites Ole Skovgaard
Green Fluorescent Protein cDNA needed Ivica Cerina
Literature needed / Biotechniques 13 Christoph
- Literature needed / Biotechniques 13 Richard Rohan
  - Literature needed / Biotechniques 13 Robert Horton
SEQUENCING Large Plasmids Michelle Larsen
lambda DNA prep Emma Macfarlane
- lambda DNA prep Martin Powell
  - lambda DNA prep the End
    - lambda DNA prep the End
Sorvall RC-24 Centrifuge Brian A. Flannigan
rna storage bolten at ubaclu.unibas.ch
expression library in mammalian cells Scott A. Coonrod
fishing out mobile elements David Demezas
M13 Phage Growth Laura K. Moen, Ph.D.
- M13 Phage Growth Warren Gallin
Wanted: Xenopus cDNA Library Ian MacLachlan
Biotech Company WWW registry NEWS martin LEACH
Literature needed / Biotechniques 13 martin LEACH
Stripping riboprobe northerns Eugene Chomey
- Stripping riboprobe northerns Aaron Pawlyk
Statistical programs sl3wx at cc.usu.edu
luminometers I am converting to luciferase from CAT assays and have only located one luminometer sold by Promega for about 6200$. Are there other models available? What other vendors sell them? Does anyone have an opinion on what to buy or about luciferase assays in general? I am using the pGL2 vectors and have bought a reagent kit from Promega. vu nguyen
- luminometers I am converting to luciferase from CAT assays and have only located one luminometer sold by Promega for about 6200$. Are there other models available? What other vendors sell them? Does anyone have an opinion on what to buy or ab Daniel Kim
- luminometers I am converting to luciferase from CAT assays and have only located one luminometer sold by Promega for about 6200$. Are there other models available? What other vendors sell them? Does anyone have an opinion on what to buy or about luciferase assays in general? I am using the pGL2 vectors and have bought a reagent kit from Promega. Philippe lefebvre
luminometers vu nguyen
- luminometers Michael Strohdeicher
Coenzyme A analogue needed koehler at ubaclu.unibas.ch
- Coenzyme A analogue needed ffdg
Secondary structure of DNA Ahmet Arman
- Secondary structure of DNA K. Legate
- Secondary structure of DNA Ann M. Yezerski
M13 Phage Growth Laura K. Moen, Ph.D.
- M13 Phage Growth ffdg
Antibody for His-Tag Flynn Lab
- Antibody for His-Tag Geraldine Butler
Two-Hybrid VP16 vector Mark Slack
Cresyl Blue for DNA ahouse
Dna Secondary structure Programs Graham Dellaire
CEM Formula!! Help, Help!! Darby Crash?
subtractive hybridisation Klaus Salger
- subtractive hybridisation Ned Mantei
primer restriction sites Andrei Popov
Does RNasin inhibit RT? Shahram Mori
Does RNasin inhibit RT? Robert Slany
- Does RNasin inhibit RT? Steven Sullivan
M13: plates are all blue! Mikhail Alexeyev
SOURCE OF VECTOR pRA1173 WHUISMAN
looking for seed company lees at SASK.USASK.CA
FACS of microbeads Louis Hom
patent of silicon carbide whisker mediated transformation lees at SASK.USASK.CA
Non-essential regions of B-lactamase gene? Nathan O. Siemers
Ampicillin resistance screening. Nathan O. Siemers
PCR problem Evan T. Keller
HELP:cloning into pGEM-T Massimo Delledonne
looking for expression vector pIN-III-lppp-5 sl3wx at cc.usu.edu
- looking for expression vector pIN-III-lppp-5 CWFisher
seeking for address lees at SASK.USASK.CA
Does RNasin inhibit RT? T. S. Grewal
acidic phenol ynakao at kean.ucs.mun.ca
- acidic phenol Richard Rohan
Problems with BRL 100 bp ladder Burnap Lab
Faint PCR Bands-Help! Eric N. Dahl
- Faint PCR Bands-Help! Richard Hastings
- Faint PCR Bands-Help! Dale Levitzke
Calling all ABI 373/377 users. Dale Levitzke
New transfection reagent Mats Carlquist
PPAR gamma M Kockx
5-Methylcytosin-mapping problem Alexander Schmitz
Faint PCR Bands-Help! Sailesh Surapureddi
Mouse strain 129 DNA Petri Kursula
2D-PAGE P. Friedl
test Carsten Jacobi
Pulse-Chase : need help ! Sandrine Imbeaud
Rat cDNA libraries - large inserts require Dr. J.P. Schofield
RNA base pairing to inosine. Richard Hastings
direct PCR from bacterial colonies. Dr. J.P. Schofield
ECL woes Rory O'Brien.
- ECL woes ssilverstone at csubak.edu
  - ECL woes Eric Anderson
production of rat antibodies against mice homologues marij at lmb1.rug.ac.be
test gks1
Separating cut from uncut vector John Dixon
- Separating cut from uncut vector Steven Enkemann
  - Separating cut from uncut vector Chris Boyd
    - Separating cut from uncut vector Steven Enkemann
    - Separating cut from uncut vector Chris Boyd
Faint PCR bands-Help! Andrei Popov
(no subject) Anders
capillary automatic sequencers Anders
None Torben ¯sterlund
Help: TLC...Diacylglycerols - Jo Brodie User Name
- Help: TLC...Diacylglycerols - Jo Brodie Kojo Mensa-Wilmot
- Help: TLC...Diacylglycerols - Jo Brodie Wolfgang Schechinger
- Help: TLC...Diacylglycerols - Jo Brodie Bernard Lassegue
12CA5 MAb immunoprecipitation Jon Backer
Hyb Solutions Rory O'Brien.
- Hyb Solutions Alan Lyons
Setting up a lab at home Susan Jane Hogarth
- Setting up a lab at home Tony Hodge
  - Setting up a lab at home Susan Jane Hogarth
    - Setting up a lab at home Tony Hodge
    - Setting up a lab at home Bill Knighton/Susan Hogarth
    - Setting up a lab at home Duncan Clark
    - Setting up a lab at home Pamela Norton
    - Setting up a lab at home Susan Jane Hogarth
inducible expression system Jaime O. Claudio
- inducible expression system Isaac Y Kim
  - inducible expression system iddavis at vms.cis.pitt.edu
Tomato cDNA library needed Corinne BOSSEUX
(no subject) Tomasz Leski
Quantitation of in vitro syn RNA Richard Rohan
Is phage display vector commercially available? Gkrue487
Electroporation of Bacteria Richard_Heath
test-please ignore jiayou Liu
HPLC Bill Zabaronick
- HPLC cbbpad
protein elution u2528082 at ucsvc.ucs.unimelb.edu.au
Glu-Glu tag Technical R&D
Genescan/Genotyper/Automated Genotyping? Tay Hou Ngee Agnes
RC/CMV sequence or Invitrogen contact? Tay Hou Ngee Agnes
help: primer restriction sites sui
help, gc rich pcr. Dale Levitzke
- help, gc rich pcr. Gilbert J. Cote
anti FYN Protein kinase Ab Giorgio Spagnol
help: primer restriction sites Clemens Suter-Crazzolara
help: crosslinking throug -COOH group at Sunderland.AC.UK
Glass beads/ acid-washed vldnaseq at hkucc.hku.hk
- Glass beads/ acid-washed Paul N Hengen
- Glass beads/ acid-washed Nancy Alexander
- Glass beads/ acid-washed Kent Nastiuk, Ph.D.
help, gc rich pcr. Steven Goldberg
How to modify QuickTake100 camera for gel documentation? Mic Chaudoir
problem with expression using baculoviruses smorgan
problem with expression using baculoviruses smorgan
problem with expression using baculoviruses Shethah Morgan
LAFMID BA PJO`B
Rnase Protection Assays rcha244 at bay.cc.kcl.ac.uk
- Rnase Protection Assays Aaron Pawlyk
- Rnase Protection Assays Francois Nantel
  - Rnase Protection Assays Jim Voeller
    - Rnase Protection Assays Pamela Norton
- Rnase Protection Assays David L. Haviland, Ph.D.
- Rnase Protection Assays Richard Rohan
  - Rnase Protection Assays Don Haut
- Rnase Protection Assays Don Haut
Rnase Protection Assays Mayumi Yagi
protein expression systems Melanie Brazil
- protein expression systems Darren Fast
Big and Bigger DNA Preps from 5 Prime # Prime Judy Leatherman
- Big and Bigger DNA Preps from 5 Prime # Prime mhughes at mbcf.stjude.org
Proving (subcloned) A549 cells are truly A549 cells Simon Twigger
problem with expression using baculoviruses Darren Fast
TnPhoA mutagenesis question Richard E. Showalter
Setting up a lab at home John Ladasky
- Setting up a lab at home Bill Knighton/Susan Hogarth
  - Setting up a lab at home Tony Hodge
    - Setting up a lab at home Susan Jane Hogarth
nonradioactive probe. Ramesh Errabolu
Vacuum pumps? Ricardo Monzon
- Vacuum pumps? Dr. Ted
mini vertical PAGE equipment Ann M. Yezerski
- mini vertical PAGE equipment A. John Watson
  - mini vertical PAGE equipment Paul C. Grimm MD
- mini vertical PAGE equipment Bill Zabaronick
- mini vertical PAGE equipment Daniel Kim
- mini vertical PAGE equipment Hiranya Roychowdhury
looking for E.coli Y1090 Ann M. Yezerski
Proving (subcloned) A549 cells... To: Nikolai
Help troubleshooting bacterial expression of prop. folded protein Joel Guthridge, Ph.D.
help needed!looking for activator for GDH enzyme HaHaHa!!!!!
Apology for advertisement Schiller01
Amplify David L. Haviland, Ph.D.
- Amplify Paul N Hengen
Definitive protocol guide? Aaron Pawlyk
- Definitive protocol guide? david l gillespie
- Definitive protocol guide? Amos Heckendorf
  - Definitive protocol guide? Mark S. Strom
Amplify Klaus Salger
How to modify QuickTake100 camera for gel documentation? The Wagner Lab
Amplify Mark S. Strom
Help needed!looking for activator for GDH HaHaHa!!!!!
Apology for advertisement Susan Jane Hogarth
urea/tmao assays Filiberto Gonzalez Prieto
DNA handling Darmono Taniwiryono
HELP, PCR OF GC RICH TEMPLATE. Dale Levitzke
- HELP, PCR OF GC RICH TEMPLATE. Daniel Mytelka
Fluoroscine dye for Ca++ ~i~
PCR primer instability Robert Brushia
DNA handling martin LEACH
Using ECL for Northerns Thomas Chuang
- Using ECL for Northerns Angela Hofstra
- Using ECL for Northerns Shaun Heaphy
Transfection of normal human cells Mukund Nori
- Transfection of normal human cells ALASTAIR MACKAY
- Transfection of normal human cells Dima Klenchin
Help on site-directed mut Harold Drabkin
Reconminant DNA conference 1996? gen at MED1.BMI.AC.CN
syngenic antibodies marij at lmb1.rug.ac.be
b-gal assay Eirikur Sigurdsson
- b-gal assay Brad Nicholson
- b-gal assay Richard E. Showalter
- b-gal assay Stephen Mamber
  - b-gal assay the End
Quantitative PCR William Alexander
URGENT - Primer for sequencing Klaus Lun
- URGENT - Primer for sequencing Paul N Hengen
- URGENT - Primer for sequencing Andreas Becker
- URGENT - Primer for sequencing Sara Contente
The DNA Vaccine Web and Protocols Robert WHALEN
DNA "refusing" to be run in agarose gel Paul N Hengen
- DNA "refusing" to be run in agarose gel Stephen R. Lasky, Ph.D.
  - DNA "refusing" to be run in agarose gel suihuang at usa1.com
power supply banan-plug splitter cable John Alsobrook
- power supply banan-plug splitter cable Duncan Clark
- power supply banan-plug splitter cable Sara Contente
- power supply banan-plug splitter cable Saurav Misra
pGL2 insert fragment sizes Cam Patterson
biotinylation of proteins R. Woodward
- biotinylation of proteins CWFisher
Bachem address Denni Schnapp
- Bachem address Jarmo Niemi
Sequence analysis software for DOS/Windows? Steven Sullivan
- Sequence analysis software for DOS/Windows? Ann M. Yezerski
in situ pcr sang at TACAPE.BF.EPM.BR
RT in situ PCR Jose Carballido
- RT in situ PCR Mary P. Remington
in situ PCR course? Kathleen Hopp
problem with expression using baculoviruses Curt Ashendel
Bachem address Joan Boyce
pGL2 insert fragment sizes varyc.mmcri at mmc.org
- pGL2 insert fragment sizes zhangxia at post.queensu.ca
1kb ladder kinase oddity. Craig M. Story
Transfection of normal human cells Kappei KOBAYASHI
apoptosis and trypsinization Robert Hewitt
- apoptosis and trypsinization P. Friedl
  - Apoptag kits in Plant tissue. Any experiences? Thorsten Jabs
ask for Primer design program running on PC tann at SCO1.MED.CUHK.HK
- ask for Primer design program running on PC Andreas Becker
- ask for Primer design program running on PC Wolfgang Schechinger
Help troubleshooting bacterial expression of prop. folded protein gen at MED1.BMI.AC.CN
Source of Cellophane for Drying Gels dinakar bhattramakki
- Source of Cellophane for Drying Gels Ruediger Hornig
Software for windows to compare sequences dinakar bhattramakki
- Software for windows to compare sequences Elizabeth Bates
Plant DNA R. Dana - Green Gene
- Plant DNA Volker Knoop
Bachem address, thanks Denni Schnapp
subtractive hybridization or differential display ? Mauro
- subtractive hybridization or differential display ? Bart Janssen
High salt/EtBr plasmid extraction: REFERENCE? Andrei Popov
KoAc R. Woodward
- KoAc Tomas Drgon
Bachem address, thanks Joan Boyce
long sequencing plates (80 cm) Keith
- long sequencing plates (80 cm) Wolfgang Schechinger
- long sequencing plates (80 cm) Wolfgang Schechinger
- long sequencing plates (80 cm) Wolfgang Schechinger
- long sequencing plates (80 cm) Wolfgang Schechinger
Chloroform Replacement? David R. Rockhold
Antibody to Human LCAT (lecithin cholesterol acyl transferase) Needed xia zhang
demonstrating protein-DNA interaction Tom Brock
detection of small changes in DNA size AJ. Murray
Jumping PCR question Bart Janssen
Chloroform Replacement? s060965 at AIX1.UOTTAWA.CA
ask for Primer design program running on PC Sergey V. Orlov
KoAc Sergey V. Orlov
- KoAc Hiranya Roychowdhury
Please help.... vishwas at bcgoa.ernet.in
(no subject) Allison Weiss
SP6 RNA polymerase -- HELP Walter Blank
- SP6 RNA polymerase -- HELP Jennifer Lyon
- SP6 RNA polymerase -- HELP "Olga Hernandez ", CHE
  - SP6 RNA polymerase -- HELP Nigel Kenward
    - SP6 RNA polymerase -- HELP Duncan Clark
  - SP6 RNA polymerase -- HELP Warren Gallin
- SP6 RNA polymerase -- HELP Richard Rohan
Leaking film cassettes jose
cloning aflp amplification products Youre-mailaddress
recombinant PKC Sylvain Bourgoin
Long Ranger Gel Mixes -- Where to Get? A. John Watson
- Long Ranger Gel Mixes -- Where to Get? Arlene Hwang
  - Long Ranger Gel Mixes -- Where to Get? gustilo at pobox.upenn.edu
- Long Ranger Gel Mixes -- Where to Get? Sara Contente
Tissue culture pipet tips Beverly Steele Allen, Ph.D.
High salt/EtBr plasmid extraction: REFERENCE? pskaytes at INTNET.UPJ.COM
- High salt/EtBr plasmid extraction: REFERENCE? Paul N Hengen
Jumping PCR question Peter Wang
Jumping PCR question Peter Wang
jumping PCR refs Peter Wang
RNA storage Kimberly Walker PhD
- RNA storage Cathy Sprankle
- RNA storage Shaun Heaphy
  - RNA storage Anton Scott Goustin
    - RNA storage Ken Pfarr
    - RNA storage Tracy Aquilla
help: primer restriction sites Kimberly Walker PhD
TnPhoA mutagenesis question Kimberly Walker PhD
- TnPhoA mutagenesis question Paul N Hengen
TnPhoA mutagenesis question Kimberly Walker PhD
EtBr filter for CCD camera? Pavel Sova
Unstable Construct Steven Enkemann
- Unstable Construct Graham Dellaire
Quantitative PCR which meets mathematical ideals Peter Doris
- Quantitative PCR which meets mathematical ideals Todd Miller - Pharmacology
  - Quantitative PCR which meets mathematical ideals Peter Doris
- Quantitative PCR which meets mathematical ideals William Alexander
Leaking film cassettes Jim Hutchins
Chloroform Replacement? Marielle Afanassieff x2832
Leaking film cassettes Dan Wells
Leaking film cassettes Dan Wells
Leaking film cassettes Dan Wells
Leaking film cassettes Dan Wells
- Leaking film cassettes Eric Anderson
Leaking film cassettes Dan Wells
Leaking film cassettes Steven Enkemann
Jone's Chromatography mailing address. Santhat Lekhavadthanajai - SCMI - 3636187
SP6 Polymerase David Figueroa
Long Ranger, where to order To: Nikolai
Efficient L929 transfection? Christoph Reinhard
- Efficient L929 transfection? Dima Klenchin
(no subject) jon boyson
RNA storage Jim Hutchins
detection of small changes in DNA size John Ladasky
- detection of small changes in DNA size Bart Janssen
detection of small changes in DNA size Dr. Ted
- detection of small changes in DNA size Chris Boyd
Opportunity - Boulder, Colorado vast
Owl Scientific Brian Scruggs
Help!! look for activator for enzyme HaHaHa!!!!!
need help Ahmet Arman
help autosequencing Juliewoods
RNA storage Zeiler, Brian M'bio
Lifting COS cells after transfection Karley Little
- Lifting COS cells after transfection martin LEACH
Universal bases in PCR primers Ron Croy
- ?Re: Universal bases in PCR primers John Sargeant
- Universal bases in PCR primers Mike Dalrymple
Universal bases in PCR primers Ron Croy
HELP! Mab agaist chicken CD? BLLIM
HELP! souce for Mab agaist Chicken CD antigen bllim at hkucc.hku.hk
b-hexosaminidase antibodies Estanis Navarro G¢mez
Multiplex PCR I.Ridgers at nhm.ac.uk
Heparin column, question to users Albert D.G. de Roos
His-tag protein purification Tomas Drgon
Hoeffer "minflourometer" Retry? Vladimir Yamshchikov
- Hoeffer "minflourometer" Retry? Dr. Chihara
RNA storage Andrei Popov
**Help** phage screening problem neale at mbcf.stjude.org
Please Help Me with My Mutations John Fox
HELP! Mab agaist chicken CD? Joan Boyce
I am looking for the bookstores which carry journals. Ken YOKORO
How to :spam removed bionet kang at msvax.mssm.edu
DNA Sequencing troubleshooting? Joseph Cook
HRP David Creely
Adding His tags to PCR products Mayumi Yagi
- Adding His tags to PCR products Maria Cristina Costanzo
Setting up a lab at home NARENDRA S YADAV
- Setting up a lab at home Steven Sullivan
  - Setting up a lab at home Jonathan B. Marder
  - Setting up a lab at home Robert Horton
    - Setting up a lab at home Kenneth HOWE
  - Setting up a lab at home Paul N Hengen
- Setting up a lab at home Bill Knighton/Susan Hogarth
arachidonic acid poster xiaokezh at kuhub.cc.ukans.edu
Can M-MuLV RT incorporate [F]dUTPs? Garry P. Larson
- Can M-MuLV RT incorporate [F]dUTPs? Zhongguo Xiong
HRP Tomas Drgon
mitochondrial DNA msautel at ulys.unil.ch
40kb Electroporation Barry Moore
NIH image-Windows version wgarrett at GGPL.ARSUSDA.GOV
- NIH image-Windows version Duncan Clark
  - NIH image-Windows version Robert Horton
How to :spam removed bionet Graham Dellaire
help: primer restriction sites Graham Dellaire
help: primer restriction sites Joanne D. Andreadis
His-tag protein purification paul bundock
- His-tag protein purification Eric Anderson
- His-tag protein purification QIAGEN
  - His-tag protein purification -correction QIAGEN
  - His-tag protein purification QIAGEN
TEST taccioli at rascal.med.harvard.edu
picking colonies help needed araizis at chmeds.ac.nz
- picking colonies help needed Madhu Singh
- picking colonies help needed A. John Watson
Quantitative PCR PATW at CLONTECH.COM
www from Techcomp- agarose Mr Eric Chan
Oligo Vital Stats/Bipartite NLS Locator Chong-Yee Khoo
Cell surface crosslinking Giorgio Spagnol
Sorbitol ANDERS_A at nsagw.nsa.bt.co.uk
Japanese-English technical terms (molbio) Gregory Stuart
Separating cut from uncut vector Mark W. Trumbore
- Separating cut from uncut vector Chris Boyd
  - Separating cut from uncut vector Klaus Bluml
Info about Trichodermin dinakar bhattramakki
40kb Electroporation Dima Klenchin
Test G. Taccioli
Inhibition of Plasma Membrane Ca++ ATPase sjp2 at bioc.cam.ac.uk
ANNOUNCEMENT: PROTEIN BIOTECHNOLOGY COURSE Andre W. Schram
Need Help!! Patrick R. Jones
- Need Help!! Aquiles Luna-Rodriguez
- Need Help!! Guy Oshiro
- Need Help!! Jami Grossfield
RC/CMV sequence or Invitrogen contact? Joan Boyce
- RC/CMV sequence or Invitrogen contact? Karl Fischer
- RC/CMV sequence or Invitrogen contact? gustilo at pobox.upenn.edu
- RC/CMV sequence or Invitrogen contact? Tokio Nakane
TCA precipitation Vladimir Yamshchikov
Hybridization Ovens Hector Lucero
- Hybridization Ovens Cathy Sprankle
- Hybridization Ovens Michael Myers
  - Hybridization Ovens Tracy Aquilla
- Hybridization Ovens Nigel Kenward
- Hybridization Ovens Cathy Sprankle
Screening the TF by southwestern method ±è¸í±â
Cell surface crosslinking mazella at naxos.unice.fr
Automatic sequencers: best choice? Daniel.Marc
- Automatic sequencers: best choice? Makino Hideshi
  - Automatic sequencers: best choice? pgegen at rnaworld.bio.ukans.edu
help: crosslinking throug -COOH group Curtis Herbert
PCR mutagenesis and primer design? Ronald Smulders
- PCR mutagenesis and primer design? Dan Deaguiar
- PCR mutagenesis and primer design? Andy Kravetz
PCR of phage library Steven Goldberg
- PCR of phage library Anton Scott Goustin
  - PCR of phage library Unsal Kuscuoglu
Dig-system Jaana Tuomainen
- Dig-system Burkhard Hassel
- Dig-system Rod Snowdon
Does CMV promoter work in murine ES Cells? John Dixon
test - don't read this! Jaana Tuomainen
Dig-Northerns Jaana Tuomainen
nucleii storage Benke/Belmar Login
Protein standards for DENATURING (9M urea) IEF Stephen P. Driska PhD
Spread sheet for quantitative PCR Andreas Becker
Protein standards for DENATURING (9M urea) IEF Nikolai Chitaev
Preprint: Amplify complete HIV-1 provirus (virtually) Mika Salminen
Protein standards for DENATURING (9M urea) IEF Tom Thatcher
T7-globin clone Tien-Hsien Chang
- T7-globin clone NICHOLAS THEODORAKIS
Good alkaline pH indicators? Saurav Misra
To Stoffel or not to Stoffel (in PCR that is) Bradley A. Arrick
- To Stoffel or not to Stoffel (in PCR that is) RACLARKASG at DELPHI.COM
protein microseqencing (summary) Tomas Drgon
Commerical Source for Tubulin Anthony Otsuka
Thanks: E. Coli Ann M. Yezerski
does glycogen interfere w/PCR? Steven Sullivan
- does glycogen interfere w/PCR? Don Haut
citation software martin LEACH
- citation software David J. Evans
Preprint: Amplify complete HIV-1 provirus (virtually) Mika Salminen
- Preprint: Amplify complete HIV-1 provirus (virtually) Robert Horton
Quantitative PCR Richard Rohan
- Quantitative PCR suihuang at usa1.com
PCR of phage library Jim Voeller
- PCR of phage library AShaw5
Germanium Michael Ian Sklar
- Germanium lonnie
- Germanium Wolfgang Schechinger
SSCP silver staining? Corinne Belville
- SSCP silver staining? bmay
time waster cDNA kit Andreas Kalmes
Earl Ruley Elution Wiggy
Help : The Alf Express automatic sequencer !! dmncli02 at uctvms.uct.ac.za
Microplate history compiled for free circulation MICROPLATE
Nasty Equilibria varyc.mmcri at mmc.org
resuspend DNA Darmono Taniwiryono
- resuspend DNA zhoufeng chen
  - resuspend DNA Tim Barnett
- resuspend DNA Sami Kohan
  - resuspend DNA Ned Mantei
- resuspend DNA Jose E. Mejia
- resuspend DNA Regina Shaw
CHO suspension Culture Nam
- CHO suspension Culture Mike Preston
- CHO suspension Culture H. David Fischer
PRIMER 0.5 jose
- PRIMER 0.5 Anderas Becker
Control human primers Duncan Clark
- Control human primers Richard Rohan
Preprint: Amplify and Clone complete HIV-1 Mika Salminen
luminometers again deborah pinney
- luminometers again martin LEACH
FAQ access Marc J Glucksman
- FAQ access Paul N Hengen
Need Help: "Blocking Primer" in PCR Christian P. Sommerhoff
Ganciclovir Cell Selection Bob Harrison
MacMolly help needed fred krebs
Need Help: "Blocking Primer" in PCR Edwin Fink
HELP: Sample storage in EtOH - Low DNA Yields? ANTHONY WILSON
- HELP: Sample storage in EtOH - Low DNA Yields? Amos Heckendorf
  - HELP: Sample storage in EtOH - Low DNA Yields? Andre Hamel
Problems cloning adaptor Bart Janssen
- Problems cloning adaptor Thomas Triolo
- Problems cloning adaptor Ben Lieberman
- Problems cloning adaptor Richard Rohan
  - Problems cloning adaptor Bart Janssen
- Problems cloning adaptor kang at msvax.mssm.edu
matrix metalloproteases Daniel Grenier
DNA purifiction methods needed R. Dana - Green Gene
hybridization Ramesh Errabolu
Rapid E. coli RNA isolation S. LaBonne
- Rapid E. coli RNA isolation Christopher M. Tebeau
Want recent, good PCR manual. James Kling
- Want recent, good PCR manual. H.J. Sluiman
- Want recent, good PCR manual. h.woo
Azide inhibition of HRP Alex Morla
- Azide inhibition of HRP BOMINAAR at chem.rug.nl
- Azide inhibition of HRP John Sargeant
- Azide inhibition of HRP simon
Strepts and Qiagen R Daines
- Strepts and Qiagen QIAGEN
Recomendation on DNA software Needed Graham Dellaire
- Recomendation on DNA software Needed Wolfgang Schechinger
CHO suspension Culture Dima Klenchin
Looking for PLA2 M. Schurdak
To Stoffel or not to Stoffel (in PCR that is) C. S. Prakash
DNA size markers "PBS::NELSONJ" at PBS.DFO.CA
- DNA size markers Craig Echt
identify mouse neural crest cell Shao-Yu Chen
seeking L-cis-Diltiazem ~i~
two gene expression vector? Grtful ned
electroporation of U937 cells Grtful ned
- electroporation of U937 cells Dima Klenchin
direct PCR amplification using library AShaw5
African Research Institutes lees at SASK.USASK.CA
help with pcr cloning AShaw5
Stupid Question regarding PCR Sami Kohan
NEED HELP - Polyagglutination Black Rose
TBE modifier Neil Van de Water
- TBE modifier Robert Horton
Precipitate in DIG Hybridization Solution? h.woo
non radioactive DNA in situ hybridization in fungi Martin Grube
Precipitate in DIG Hybridization Solution? Rosanne Casu
Batteries for Rainin pipetteman? G.P.
- Batteries for Rainin pipetteman? Sarah K. Highlander
transposon mutagenesis micjms at lure.latrobe.edu.au
(no subject) Jorge Blackhall
two gene expression vector Graham Atherton
PRIMER 0.5 Gregory M. Acland
ligase reaction/how long ? Clemens Suter-Crazzolara
- ligase reaction/how long ? David Micklem
- ligase reaction/how long ? Tomas Drgon
- ligase reaction/how long ? zhoufeng chen
  - ligase reaction/how long ? Morrison Lab
- ligase reaction/how long ? Burkhard Hassel
Usefulness of 96 -well PCR Sabah
- Usefulness of 96 -well PCR Deb Britt
  - Usefulness of 96 -well PCR Andy Law Big Nose
- Usefulness of 96 -well PCR Craig Echt
luciferin-esters and flow cytometry? David de Graaf
- luciferin-esters and flow cytometry? Garry P. Nolan
monitoring promotor activity in individual live cells? David de Graaf
- monitoring promotor activity in individual live cells? Garry P. Nolan
  - monitoring promotor activity in individual live cells? Richard R. Hardy
Whic PCR machines work for Long PCR Duncan Clark
- Whic PCR machines work for Long PCR Ted M.
  - Whic PCR machines work for Long PCR Duncan Clark
test DR. KEITH BONHAM
Looking for Mycobacterium marinun strains Bob Belas
Stupid Question regarding PCR Richard Rohan
- Stupid Question regarding PCR Robert Horton
Contact for Cambridge Isotopoes in UK? Dr Graham Timmins
African Research Institutes C. S. Prakash
Best kit for RPA sabah
- Best kit for RPA A. John Watson
- Best kit for RPA Richard Rohan
Antipeptide antibodies LOGAND
- Antipeptide antibodies Neal Freedman
labeling oligos for FISH Scott Cain
earn Swiss chocolate: a tough RT-PCR / RAPD nut VOGEL at ubaclu.unibas.ch
electroporation of U937 cells RVDESHP0 at PWINET.UPJ.COM
NIH Image for windows Wolfgang Schechinger
Kit for Beta-gal staining rx86270 at mcvax2.d48.lilly.com
- Kit for Beta-gal staining Pamela Norton
Batteries for Rainin pipetteman? Wolfgang Schechinger
Batteries for Rainin pipetteman? Wolfgang Schechinger
Contact for Cambridge Isotopoes in UK? Joan Boyce
Contact for Cambridge Isotopoes in UK? Joan Boyce
PCR-SSCP with 33P ynakao at kean.ucs.mun.ca
EtBr filter for CCD camera? Hemant Chickarmane
- EtBr filter for CCD camera? Robert Horton
- EtBr filter for CCD camera? John Tudor
- EtBr filter for CCD camera? Christopher B. Yohn
symposia on line varyc.mmcri at mmc.org
looking for lacZ gene with NdeI site at ATG A.P.N. de Boer
Strep, Staph and coli PCR templates John Wm. Tobias
2 hybrid system and IF proteins jfh
SSCP gel smiling problem Greg Niemi
- SSCP gel smiling problem Daniel Kim
- SSCP gel smiling problem Alberto Catalano
  - SSCP gel smiling problem Jim Voeller
Probes for Hanta virus? Gordon Betts
Asymmetric PCR Richard Friedman
- Asymmetric PCR donal at gene.genetics.uottawa.ca
- Asymmetric PCR Michael J. Tuvim
- Asymmetric PCR Wolfgang Schechinger
Human Bcell culture Gavin Fischer
- Human Bcell culture Anton Scott Goustin
What's the skinny on ALF vs LI-COR? ajeffs at chmeds.ac.nz
- What's the skinny on ALF vs LI-COR? Wolfgang Schechinger
- What's the skinny on ALF vs LI-COR? pgegen at rnaworld.bio.ukans.edu
- What's the skinny on ALF vs LI-COR? pgegen at rnaworld.bio.ukans.edu
  - What's the skinny on ALF vs LI-COR? Duncan Clark
? about mtDNA isolation Juliewoods
RPA or Northerns???? Melissa Hill
- RPA or Northerns???? Tracy Aquilla
protocol for cutting IgG sophia
Please repost boiling miniprep method Kristin B. Andersson
- Please repost boiling miniprep method Mike Dalrymple
  - Please repost boiling miniprep method Karl Fischer
348 kD protein isolation and purification. Danilo Tait
Thermocycler(s) for long range PCR... rhubner at molbiol.ox.ac.uk
Taq errors Stephen Perry
- Taq errors Paul N Hengen
- Taq errors Peter Kaplan
Salmon cDNA expression libs tjames
Drop Dialysis of Ligations Rich Kernan
- Drop Dialysis of Ligations Mark Siegal
  - Drop Dialysis of Ligations Christian Fritze
  - Drop Dialysis of Ligations the End
    - Drop Dialysis of Ligations Mark Siegal
Please repost boiling miniprep method Tomas Drgon
anatomy www site Clemens Suter-Crazzolara
348 kD protein isolation and purification. Dima Klenchin
Taq errors Isaac Y Kim
antibody against eIF2 alfa subunit Jorge Blackhall
RPA or Northerns???? Isaac Y Kim
- RPA or Northerns???? Richard Rohan
best oligo end-labeling procedure Sergey V. Orlov
Picornavirus ahung at ASRR.ARSUSDA.GOV
Tawn serum Luc Camoin
looking for an enzyme activator HaHaHa!!!!!
Drop Dialysis of Ligations Stephen P Lee
FAQ Mungo Marsden
5' RACE Michael Perry
Branched DNA Ash
- Separation dsDNA and ssDNA ToxicTish
- Branched DNA Cathy Spargo
- Branched DNA Cathy Spargo
plasmid gene orientation? david marcey
Plant Transformation Vector Needed Rick Davis
mRNA isolation questions Edward Wang
- mRNA isolation questions Jim Hutchins
Hot PCR Jeff Sale
- Hot PCR Klaus Salger
- Hot PCR Brian Grimwade
  - Hot PCR Robert Horton
Who sells 96 well dispensers? Jill Maddox
5-oxytryptophane Michael Ian Sklar
ANNOUNCEMENT: Molecular Recognition and Signalling. Lennart Nilsson, KI/CSB
pcr primers for cancer antigen 125 wanted Thomas Mohr
looking for bovine actin probe T. S. Grewal
DNA from clotted blood Jim Voeller
Site-Directed Mutagenesis Kits Jami Grossfield
- Site-Directed Mutagenesis Kits theveno at cc.umanitoba.ca
- Site-Directed Mutagenesis Kits Andy Kravetz
  - Site-Directed Mutagenesis Kits Robert Horton
    - Site-Directed Mutagenesis Kits Andy Kravetz
    - Site-Directed Mutagenesis Kits Robert Horton
- Site-Directed Mutagenesis Kits Chris Ferrer
- Site-Directed Mutagenesis Kits John K. Troyer
- Site-Directed Mutagenesis Kits vipond at bsa.bris.ac.uk
Separation dsDNA and ssDNA ToxicTish at aol.com
Want recent, good PCR manual. Rebecca Schall
MiniCycler??? solcm at post.queensu.ca
- MiniCycler??? J. David Spafford
- MiniCycler??? gerald forrest
  - MiniCycler??? Bill Knighton/Susan Hogarth
    - MiniCycler??? Ted M.
- MiniCycler??? benedik at uh.edu
Sinthetic peptides manufacturer Giorgio Spagnol
- Sinthetic peptides manufacturer Nigel Kenward
- Sinthetic peptides manufacturer John E. Fox
Help in Western blot Giorgio Spagnol
- Help in Western blot John K. Troyer
  - Help in Western blot Massimo Delledonne
Site-Directed Mutagenesis Kits Karen.Straube-West
- Site-Directed Mutagenesis Kits Richard E. Showalter
Batteries for Rainin Pipettor Sarah K. Highlander
MALDI Mass Spec review ladroru at randc.pprd.abbott.com
Hot PCR Andrei Popov
Sequencing shareware Matthew P. Frosch, MD, PhD
- Sequencing shareware gustilo at pobox.upenn.edu
Want to buy GSA bottles Sanjay Krishnaswamy
- Want to buy GSA bottles Ted M.
binding help need! sang at TACAPE.BF.EPM.BR
- binding help need! Steve Mcclue
bovine primer information Kariman Dadbeh
Where to get ethephone kloska at mpimg-berlin-dahlem.mpg.de
Help with PCR primers olssonl at spot.colorado.edu
Storage buffers for DNA Cheryl Dean
long shelf-life of THC (marijuana) in human urine Shelley Cole
||||||||||||||||||||Looking for Import Traders||||||||||||||||||| Ken YOKORO
Help in Western blot skim at ALLIANT.SNU.AC.KR
Anecdotes/stories on the internet and your science/research Dylan Bulseco
Asymmetric PCR AShaw5
JENNIFER LYON Ash
Ambion's Nonisotopic Ssytem Eric Hanson
looking for expression vector pIN-III-lppp-5 kang at msvax.mssm.edu
ZnCl Phage Precipitation? the End
- ZnCl Phage Precipitation? o. van-ham
Cell Culture porous membranes Balpat
- Cell Culture porous membranes U12201 at uicvm.uic.edu
  - Cell Culture porous membranes Angela Hofstra
- Cell Culture porous membranes MICROPLATE
Cell Culture porous membranes Dima Klenchin
inducible promoter Fen Zhang
glassine paper garrell
Sequencing gel size marker dyes Jeffrey Keen
- Sequencing gel size marker dyes Robert Horton
site-directed mutagenesis kits Rich Kernan
test-ignore bwr at icbr.ifas.ufl.edu
purification of protein from exclusion bodies DENNIS S. FRANCE
- purification of protein from exclusion bodies Wulf Blankenfeldt
- purification of protein from exclusion bodies Jennifer Lyon
Test/ignore PRETORIUS
Blunt-end Ligation PRETORIUS
- Blunt-end Ligation gene cutler
- Blunt-end Ligation Nigel Kenward
  - Blunt-end Ligation the End
- Blunt-end Ligation Earl J. Gubbins
Protease deficient hosts for expression Peter Carlsson
- Protease deficient hosts for expression LKM100F at ODUVM.BITNET
competent cells/antibiotics PRETORIUS
***FMC kits?**** b.schonhoft
Commercial source for archaea? Malcolm White
- Commercial source for archaea? Duncan Clark
Luminometer software Bjšrn Olde
Why acetic acid for Coomassie? LOGAND
Influenza HA1 epitope erty
- Influenza HA1 epitope Anne Spang
HSP70 PROMOTER WANTED ! damania
- HSP70 PROMOTER WANTED ! Steve
ZnCl Phage Precipitation? (fwd) Jinny Lin
post ladroru at randd.pprd.abbott.com
HortScience Address lees at SASK.USASK.CA
Why acetic acid for Coomassie? Hiranya Roychowdhury
- Why acetic acid for Coomassie? Duncan Clark
used equipment Bob Gross
- used equipment H.J. Sluiman
degenerated primers PCR HELP!!!! Daniele Eusebe
Problem with 5-FOA plates. Kenneth Shaw
Plasmid drawing software? Dr. Gusscow
Custom DNA sequencing Michael Kertesz
expression vector Benke/Belmar Login
- expression vector Nigel Kenward
RNA PCR primers Robert Horton
AGAGAGAG repeats:Could it be real? Changcun Jia
- AGAGAGAG repeats:Could it be real? John Ladasky
- AGAGAGAG repeats:Could it be real? Massimo Delledonne
- AGAGAGAG repeats:Could it be real? Massimo Delledonne
- AGAGAGAG repeats:Could it be real? John Greally
  - AGAGAGAG repeats:Could it be real? Beth Wapelhorst
    - AGAGAGAG repeats:Could it be real? Dale Levitzke
HortScience Address C. S. Prakash
(no subject) Hectoe Lucero
HELP with agarose gel m
- HELP with agarose gel LouPass
CEM - HELP, HELP!! Darby Crash?
Glycogen versus RT! Darby Crash?
- Glycogen versus RT! Duncan Clark
  - Glycogen versus RT! Duncan Clark
Plasmid drawing software? the End
lab animal care varyc.mmcri at mmc.org
Whole Mount Immunohistochemistry DJ Kane
- Whole Mount Immunohistochemistry jim crouch
- Whole Mount Immunohistochemistry jim crouch
in vitro transcription problem Keith Hoek -BIOCHEM
- in vitro transcription problem Edwin ten Dam
- in vitro transcription problem Rizaldy P Scott
  - in vitro transcription problem Pamela Norton
Looking For Standard Molecular Bio lab Practices Sandra Poudrier
in vitro transcription problem Zeiler, Brian M'bio
"recycling" Qiagen plasmid prep columns John Bradsher
- "recycling" Qiagen plasmid prep columns martin LEACH
  - "recycling" Qiagen plasmid prep columns nathalie campbell dyke
  - "recycling" Qiagen plasmid prep columns Amos Heckendorf
- "recycling" Qiagen plasmid prep columns "Marianne Leverone ", BIO
  - "recycling" Qiagen plasmid prep columns John Sargeant
- "recycling" Qiagen plasmid prep columns Christian Velten
DIG Gel Shift Kits ? Emma T Tonkin
Blunt end ligations HARDIES at UTHSCSA.EDU
ligase reactions/how long? HARDIES at UTHSCSA.EDU
restriction mapping Alyson Peel
Test post - Ignore David Atherton
Help with GRE ralcon at ibm.net
- Help with GRE Tracy Aquilla
- Help with GRE mhughes at mbcf.stjude.org
Beckman Biomek 2000 David Atherton
IS IT OK TO LIGATE AT RT OVERNIGHT? rew
- IS IT OK TO LIGATE AT RT OVERNIGHT? Martin Kennedy
  - IS IT OK TO LIGATE AT RT OVERNIGHT? Graham Dellaire
Promoter CAT assays Alec Redwood
Northern Blot - METHYLENE BLUE Cathy Sprankle
What is TALON metal Yasushi OKADA
Phosphorylated tBOC-AA derivates? Andrey Mikhailow
"Glassmilk" Mungo Marsden
Help with GRE Sami Kohan
Northern Blot - METHYLENE BLUE Dirk
Agarose Gel run problem TAN HUI HUI
Specific peptide phosphorylation? Michael Daws
Problem with REP and ERIC primers TAN HUI HUI
- Problem with REP and ERIC primers 4700gbera at umbsky.cc.umb.edu
UK Distributor for Molecular Probes Inc. Duncan Clark
Calling Macintosh users Graham Atherton
- Calling Macintosh users Tony Hodge
- Calling Macintosh users Bart Janssen
problem with 5-FOA plates Andrei Popov
freeze-drier for HPLC fractions Denni Schnapp
- freeze-drier for HPLC fractions Tom Thatcher
- freeze-drier for HPLC fractions Peter Barling
freeze-drier for HPLC fractions Wolfgang Schechinger
Long PCR from lambda phage clone Jim Voeller
DNA isolation for Francisella needed chengs1 at jaguar.uofs.edu
transfectable, LPS-sensitive cell line Gill Diamond
Calling Macintosh users Jim Woodgett
- Calling Macintosh users Darby Crash?
  - Calling Macintosh users Jim Woodgett
ANNOUNCE: The CYTOKINE WEB site Simon M. Brocklehurst
- Amphioxus cDNA library? Tomas Bratt
GST cellular localization Scott Vande Pol
need help on acid-plate screening Tao Wei
Need pMT2 (3) vector seq from Genetic Ins, MA hong dang
freeze-drier for HPLC fractions John K. Troyer
- freeze-drier for HPLC fractions Rajesh Krishnamurthy
sequencing pcr product with 35S Igor R Sagdeev
Two questions Igor R Sagdeev
- Two questions Rafael Maldonado
macrophage toxic diezrg01 at mcrcr6.med.nyu.edu
- macrophage toxic Ted Michelini
Two questions Ted Michelini
Long PCR from lambda phage clone Ted Michelini
Review of Mass Specs. ladroru at randd.pprd.abbott.com
Help with GRE Ted Michelini
sequencing pcr product with 35S Ai-Li Shiau
do not ligate at RT for O/N Burnap Lab
- do not ligate at RT for O/N Mad Dan Eccles
- do not ligate at RT for O/N the End
- do not ligate at RT for O/N Ted Michelini
PCR with 7-deaza-2'-deoxyguanosine triphosphate galloway at dogwood.botany.uga.edu
- PCR with 7-deaza-2'-deoxyguanosine triphosphate Dale Levitzke
- PCR with 7-deaza-2'-deoxyguanosine triphosphate Dale Levitzke
transfection problems jon boyson
(no subject) Marilene Guimaraes
Calling Macintosh users Ian A. Boussy
- Calling Macintosh users Klaus Eimert
E. coli MOSblue strain Marilene Guimaraes
labelling DNA for screening RMCMAH93 at IRLEARN.UCD.IE
IS IT OK TO LIGATE AT RT OVERNIGHT? Volker Knoop
mRNA purification Edward Wang
Marathon-Ready cDNAs - Good or bad? Torsten Nielsen
Qiagen on the Web? Not! martin LEACH
Two questions martin LEACH
Taq errors ANTHONY WILSON
IS IT OK TO LIGATE AT RT OVERNIGHT? jim hartley
?'s about a good PCR polymerase Brian Butler
Northern Blot - METHYLENE BLUE H. Yim
IS IT OK TO LIGATE AT RT OVERNIGHT? Kenneth HOWE
- IS IT OK TO LIGATE AT RT OVERNIGHT? Stephen R. Lasky, Ph.D.
Ultra-filtration problems W.S. Jongman
program for restriction mapping Dirk Konz
Is it OK to ligate at RT overnight? Andrei Popov
- Is it OK to ligate at RT overnight? Paul N Hengen
Extinction Coefficient for benzoylarginine nitroanilide Net Pimp
Extinction Coefficient of Cephalosporin C WM
casein kinase II constructs Richard Poulin
Beckman Biomek 2000 protocol archive Mr. D.J. Atherton
Marathon cDNA for PCR nathalie campbell dyke
P1 genomic clones -HELP!!!! nathalie campbell dyke
- P1 genomic clones -HELP!!!! Kah Tong, Seow
Request for Info Vladimir Yamshchikov
Commercial kits for competitive PCR ? Thomas Haenel
heraeus e-mail? suleyman aydin
GST cellular localization Sailesh Surapureddi
HELP!!!! Digestion of primers with internal Not1 sites B VISSER * x2818 Plantkunde *
- HELP!!!! Digestion of primers with internal Not1 sites Martin Kennedy
Cloning into bacillus (Bt) PRETORIUS
Clontech promoterfinder kit Dr Mick Jones
- Clontech promoterfinder kit Jon Nakamoto
ATP Biometer sjp2 at bioc.cam.ac.uk
Isotopically labelled media D. Hodges
Using Pwo DNA polymerase for blunting. Aart A. van Apeldoorn
Problem with PCR! Just recieving smear Anja Berger
mRNA purification Anne Spurkland
Elution of RNA Steven Tait
Methyl Guanine Assay Hector Lucero
V8 protease Ralph Schuster
- V8 protease U12201 at uicvm.uic.edu
HPLC software Russell L. Malmberg
- HPLC software Dag Stenberg
- HPLC software Micro-Titre Plate Reader
Hot Start PCR jose
- Hot Start PCR Christopher Coward Path
- Hot Start PCR T. S. Grewal
rRNAs cross-hybridization problems? marina.mostardini at itner.pharmacia.se
Request: suggetions for restriction- site analysis program (pc/win) Niels Groot
ATP Biometer sjp2 at bioc.cam.ac.uk
Anyone using a Quicktake 150 with their microscope? Rick Willis
- Anyone using a Quicktake 150 for EtBr gels? jane eason
RDA-PCR Johan Lundkvist
Ligations. Do them o/n. Mad Dan Eccles
Beta-Gal Adenovirus Markus Reiser
- Beta-Gal Adenovirus Page Briscoe
Gilson pipetter calibration protocol wanted. Bruce McDonald Lab
PCR kits to detect animal viruses & bacteria (fwd) Anyname
coating with avidin Torsten Boerchers
- coating with avidin U12201 at uicvm.uic.edu
Agarose Gel run problem Sailesh Surapureddi
Source of antibiotic protein colicin E1 spehr at mail.rz.uni-duesseldorf.de
protein precipitation Tomas Drgon
- protein precipitation Page Briscoe
  - protein precipitation Tomas Drgon
    - protein precipitation Page Briscoe
- IPTG Fung Ming Chiu
  - IPTG Francisco G da Nobrega ib - bio 7588
    - IPTG Terence
- protein precipitation Anne Spang
Hot Start PCR Frederick Garbrecht
- Hot Start PCR Beth Wapelhorst
Mus cervicolor or Mus cookii DNA HARDIES at UTHSCSA.EDU
Antibodies against EF-2 Anxo (Fisioloxia)
help isolating pTrxFus from GI724 James Kling
Gilson pipetter calibration protocol wanted. martin LEACH
Alternative to Glogos Autorad Markers dinakar bhattramakki
Reuse of Quiagen columns Hernan Espinoza
Alternative to Glogos Autorad Markers Warren Gallin
- Alternative to Glogos Autorad Markers Roger Anderson
Trouble with Glycerol stocks of DH5alpha Tony Cavallaro
- Trouble with Glycerol stocks of DH5alpha Duncan Clark
- Trouble with Glycerol stocks of DH5alpha John R. Thompson
PCR-ELISA info requested Xiu Yu
- PCR-ELISA info requested Angela Hofstra
Isolating DNA-RNA hybrids Robyn Wallace
Hot Start PCR Ted Michelini
MOL. BIO. SOFTWARE FOR SILICON GRAPHICS COMPUTER? HOPE
- MOL. BIO. SOFTWARE FOR SILICON GRAPHICS COMPUTER? Tom Duncan
Using Pwo DNA polymerase for blunting. Ted Michelini
- Using Pwo DNA polymerase for blunting. evan behre
  - Using Pwo DNA polymerase for blunting. John Dixon
    - Using Pwo DNA polymerase for blunting. Duncan Clark
  - Using Pwo DNA polymerase for blunting. Aart A. van Apeldoorn
    - Using Pwo DNA polymerase for blunting. Duncan Clark
mini-preps: sodium iodide vs. guanidinium Peteris Prusis
- mini-preps: sodium iodide vs. guanidinium Michael Szardenings
  - mini-preps: NOT kits. Mad Dan Eccles
    - mini-preps: NOT kits. Michael Szardenings
    - mini-preps: NOT kits. Daniel Kim
Transfection Method Rat Hepatocytes? Martijn Groenink (expegen1)
- Transfection Method Rat Hepatocytes? Mike Roper
E.coli strain B834(DE3) requested gen at MED1.BMI.AC.CN
AT Biochem Address Neil Harris
Recombination News Group VOTE Dellaire, Graham
Ribosomal RNA primers rcha244 at bay.cc.kcl.ac.uk
Bronwill Biosonic III Help Needed Tim Uphoff
PC12 cells in Serum Free Media? Mike Herron
ligation problems egorham at beach.utmb.edu
- ligation problems Aart A. van Apeldoorn
  - ligation problems Karl Voss
Antibodies against EF-2 Kent Nastiuk, Ph.D.
SSCP questions Frederick Garbrecht
- SSCP questions Lana Perko
Help wanted with blunt ligations (molecular biology) Aart A. van Apeldoorn
- Help wanted with blunt ligations (molecular biology) John K. Troyer
- Help wanted with blunt ligations (molecular biology) William D. Woolfolk
Problems with Pseudomonas putida minipreps Jason Brinck
Gene Releaser Martin J. Kullen
fat bellossi
PC12 cells in Serum Free Media? TJ Avila
TdT with DNA sequencing HARDIES at UTHSCSA.EDU
- TdT with DNA sequencing Benoît Grondin
- TdT with DNA sequencing Benoît Grondin
  - TdT with DNA sequencing David Micklem
Blunt lig. help (NL) Jillian Johnston
- Blunt lig. help (NL) Aart A. van Apeldoorn
  - Blunt lig. help (NL) Page Briscoe
  - Blunt lig. help (NL) Aart A. van Apeldoorn
  - Blunt lig. help (NL) Danuta Bois
    - Blunt lig. help (NL) David L. Haviland, Ph.D.
    - Blunt lig. help (NL) Danuta Bois
fat Fengyu Shu
IEC MPR-4 Centrifuge Denise Lepley
Looking for anti-Luciferase antibodies Joe Walker
- Looking for anti-Luciferase antibodies martin LEACH
TdT with DNA sequencing Margon Vandongen
Two-Hybrid System Larry Fliegel
test Luis M. Alvarez
test Luis M. Alvarez
SP6 RNApol THANK-YOU Panhead McNipper
fish Benoit Auperin
fish Benoit Auperin
2-hybrid system false positives Michael Kyba
cDNA library from rat brain?! Stephan Witte
"recycling" Qiagen plasmid prep columns Bradley Turner
- "recycling" Qiagen plasmid prep columns the End
  - "recycling" Qiagen plasmid prep columns Duncan Clark
- "recycling" Qiagen plasmid prep columns Hernan Espinoza
Where to go for chromosomal localization of a gene? Bradley Turner
radiolabelled n-decane Jan Kostal
Negative staining Kirsten Blake
- Negative staining P. Friedl
Calling all Mac users Graham Atherton
Best T4 ligase b.schonhoft
- Best T4 ligase Regina Shaw
MJ Research 100 microliter rxn? J.Carnwath
- MJ Research 100 microliter rxn? Kent Nastiuk, Ph.D.
- MJ Research 100 microliter rxn? Mike Finney
Non radioactive labelling and detection rcha244 at bay.cc.kcl.ac.uk
- Non radioactive labelling and detection John K. Troyer
  - Non radioactive labelling and detection Rachel Fernandez
- Non radioactive labelling and detection Angela Hofstra
- Non radioactive labelling and detection h.woo
GFP as a reporter for two-hybrid? Daniel Durocher
Specific peptide phosphorylation? Dima Klenchin
protein purification software varyc.mmcri at mmc.org
- protein purification software John A. Newitt
Heme oxygenase-1 antibodies Cam Patterson
Search BioTechniques Database Online MMCCARTHY at BIOTECHNET.COM
- Search BioTechniques Database Online martin LEACH
  - Search BioTechniques Database Online jemerton at delphi.com
Gilson pipetter calibration protocol wanted. Curt Ashendel
source of amphioxus cDNA? Tomas.Bratt at medkem.lu.se
LDH in cell culture Heiko Alfke
Non radioactive labelling and detection Annick Wilmotte
HELP!!! type III collagen BIOSCIPA at IPV85.UNIPV.IT
Quantitative PCR David Tak Yan Yu
- Quantitative PCR Larry Fliegel
- Quantitative PCR Dave Knorr
HELPÙ EtBr disposal Mayumi Yagi
70%? DON ELLIS, II
- 70%? Rae Nishi
  - 70%? Jim Voeller
  - 70%? Lisa Vawter
HELPÙ EtBr disposal Shelley Cole
Phosphopeptide TLC software Joseph M. Lee
UDG cloning Dufton Christopher
ligation problems Sailesh Surapureddi
- ligation problems Andreas Enger
  - ligation problems tracy a davis
- ligation problems Laurie Hassell
HELPÙ EtBr disposal TORRES at vm.ci.uv.es
- HELPÙ EtBr disposal Michael Szardenings
LDH in cell culture Rae Nishi
Does CE membrane bind DNA? Shiao Y. Wang
IPTG Sergey V. Orlov
Quantitative PCR Jack Miller
- Quantitative PCR Anderas Becker
non-radioactive marker enzyme assays Torsten Boerchers
plasmid labelling with tritiated thymidine Allin Verreault in Stillman lab
- plasmid labelling with tritiated thymidine vipond at bsa.bris.ac.uk
Chemiluminescence / blots: Lumiphos 530 vs. CDP-STAR Richard Vogt
ALF EXPRESS or LIQUOR 4000? Herta Steinkellner
Best T4 ligase Sailesh Surapureddi
Perplexing plasmid problem!!?! hliber at hsph.harvard.edu
- Perplexing plasmid problem!!?! Beth Wapelhorst
TEST-IGNORE!! home/hilli
Bacterial adhesins, fimbriae etc Thomas Cook
duplicate ORI plasmid? h9290184 at hkucc.hku.hk
duplicate ORI construct? h9290184 at hkucc.hku.hk
cDNA library of BC3H1 & L6 myotubules Keith Gough
Non radioactive labelling and detection Robyn Wallace
CPG MEMBRANE DNABILL
DNA staining - help Michael Goodin
differential molecular screening L. Bastian
polyclonal antibodies: help! Giorgio Spagnol
- polyclonal antibodies: help! John K. Troyer
Help explain my DNA bands smearing jene
- Help explain my DNA bands smearing Elizabeth Bates
- Help explain my DNA bands smearing Rafael Maldonado
  - Help explain my DNA bands smearing Elizabeth Bates
- Help explain my DNA bands smearing Michiaki Masuda
- Help explain my DNA bands smearing Ron Goto x2832
- Help explain my DNA bands smearing Anne Spang
- Help explain my DNA bands smearing ryan at mbcf.stjude.org
dut- ung- bug Duncan Cochran
MutS, L, H protein source Dennis Summerbell
BL21 (DE3) Induction J.P. Hays
- BL21 (DE3) Induction, I am using this strain now. Burnap Lab
- BL21 (DE3) Induction Shaun Heaphy
- BL21 (DE3) Induction Shaun Heaphy
- BL21 (DE3) Induction Shaun Heaphy
- BL21 (DE3) Induction Shaun Heaphy
- BL21 (DE3) Induction Shaun Heaphy
  - BL21 (DE3) Induction Albert Crescenzo
Help with Shrimp Alkaline Phosphatase Juergen Sasse (Path)
- Help with Shrimp Alkaline Phosphatase Brad Nicholson
mouse intestinal cDNA expression library Dr. M Raabe
Looking for anti-luciferase antibodies rx86270 at mcvax2.d48.lilly.com
Postdoc position avaiable Andrew Matus
Oligonucleotide staining Juergen Sasse (Path)
- Oligonucleotide staining Russ Boggs
- Oligonucleotide staining Brian Grimwade
  - Oligonucleotide staining Richard Rohan
- Oligonucleotide staining Garry P. Larson
purification of 100 bp fragments James Warren Lafollette
- purification of 100 bp fragments bio.tamu.edu
- purification of 100 bp fragments Elizabeth Bates
- purification of 100 bp fragments Paul N Hengen
- purification of 100 bp fragments Dr I.M. Clark
polyadenylation positions steven morse
- polyadenylation positions Daniel R. Schoenberg
Help with RACE Sandra Munro
RT-PCR Peter Nilsson
deoxy-galactose anomaly Chris Skory
Anti-RuBISCO LOGAND
- Anti-RuBISCO Usuario Invitado
Oligonucleotide staining John K. Troyer
freeze-drying HPLC fractions Denni Schnapp
smeary PCR problem solved! Thanks for all the help! Anja Berger
Help subcloning large PCR product Russ Carston
Killing Taq jim hartley
- Killing Taq Robert Horton
duplicate ORI construct Andrei Popov
need: a drug interacting with ss or ds DNA in vitro dima at la.asu.edu
NEED HELP with non-denaturing IEF Aye M.Tin
1978 maniatis library Marci Swede
staining the cytoskelethon: help! Giorgio Spagnol
- staining the cytoskelethon: help! Wolfgang Schechinger
Help explain my DNA bands smearing Knepper, Marc A
Advice Regarding Expression Systems Ed Madison
help -- BSA for Turbo buffer? Juliewoods
- help -- BSA for Turbo buffer? Bernard Murray
- help -- BSA for Turbo buffer? A. V. Furano
HELP- tobacco GST antibodies lingyu zhang
Help explain my DNA bands smearing Robyn Wallace
- Help explain my DNA bands smearing lingyu zhang
  - Help explain my DNA bands smearing Shelley Cole
  - Help explain my DNA bands smearing Petri Kursula
UK Distributor for Molecular Probes Inc. ICleme1659
help for plasmid curing todd d pack
software for detecting introns? Steven Sullivan
- software for detecting introns? Sean Eddy
- software for detecting introns? Karl A. Suiter
Can you use a DNA ladder in a MOPS gel? Steven Sullivan
Refolding a GST-fusion-protein --- Help needed!! Wulf Blankenfeldt
antibodies to muscle proteins (Cnidaria) Jens Gundermann
Help subcloning large PCR product Andrei Popov
ExSite PCR-based mutagenesis kit Ludwig Krabben
thermostable reverse transcriptase Michael Kertesz
- thermostable reverse transcriptase Dan Baggott
- thermostable reverse transcriptase martin LEACH
- thermostable reverse transcriptase John Dixon
Papers onWhole mount Immunochemistry Jfloring at aol.com
antibodies to integrins needed Shao-Yu Chen
cAMP ASSAY KRIS B
- cAMP ASSAY Andy
Techne's "Progene" thermocyclers Jean.Mariaux at zool.unine.ch
Antibody marker of plant Golgi? LOGAND
Home-Made TA cloning David D Morgan
- Home-Made TA cloning gerald forrest
- Home-Made TA cloning Lauryl Maiya J Nutter
test posting please ingore - signatur.pce (0/1) David D Morgan
test posting please ingore - signatur.pce (1/1) David D Morgan
ABI automated sequencer caetano at FAUNA.UCDAVIS.EDU
- ABI automated sequencer Dr. J.P. Clewley
  - help with poly(A)+ mRNA purification Karen M. Page
- ABI automated sequencer Richard R. Hardy
DIG block??? Kim Snowden
software for detecting introns? Yun-Long Du, Ph.D.
Sma I digestion of genomic DNA cok at mole.bio.cam.ac.uk
what transfection internal positive control? jfh
Sma I digestion of genomic DNA Johan Burger
- Sma I digestion of genomic DNA Karl Worm
- Sma I digestion of genomic DNA Barry Moore
ECL western blot yhao at kean.ucs.mun.ca
- ECL western blot John K. Troyer
- ECL western blot Nigel McMillan
differential display HELP! at
Blunt Ligations S.L.Rennie
breaking biotin-streptavidin interaction Doug Craig
- breaking biotin-streptavidin interaction Gérard Klein
- breaking biotin-streptavidin interaction U12201 at uicvm.uic.edu
- breaking biotin-streptavidin interaction fred
Easiest/fastest ways to isolate bacterial genomic DNA? Igor R Sagdeev
- Easiest/fastest ways to isolate bacterial genomic DNA? jfh
- Easiest/fastest ways to isolate bacterial genomic DNA? Sean Eddy
Quiagen column - reuse Jaishree Muppala Chittoor
- Quiagen column - reuse John K. Troyer
priming mice for ascites Frederick Garbrecht
GE200/electroelution unit Ikuo Wada
- GE200/electroelution unit Malcolm Moos Jr., M.D., Ph.D.
Molecular biology software Mary Boyle
- Molecular biology software dinakar bhattramakki
thermostable reverse tra Jose Carballido
Molecular biology software Robyn Wallace
Getting rid of Taq polymerase, HELP Petri Kursula
- Getting rid of Taq polymerase, HELP Massimo Delledonne
- Getting rid of Taq polymerase, HELP Anne Spurkland
- Getting rid of Taq polymerase, HELP C.S.Wilding
- Getting rid of Taq polymerase, HELP jim hartley
- Getting rid of Taq polymerase, HELP Robert Horton
Capillary electrophoresis of PCR products Adrian
dam - bacterial strain Mark Mellon
- dam - bacterial strain Brad Nicholson
- dam - bacterial strain Duncan Clark
- dam - bacterial strain Bernard Murray
Rf: Re: Anti-RuBISCO LOGAND
Staining of inclusion bodies?? Frank Hoffmann
PCR with Poly A primer Elizabeth McGraw
Supplier?: Chlorazol Paper Brown B Michael Deyholos
Kinase site database? Graham Atherton
Storing Northerns Eric C. Anderson
Oligo probes and Northerns Mr. S.M. Jones
- Oligo probes and Northerns pgegen at rnaworld.bio.ukans.edu
ExSite PCR-kit from stratagene Ludwig Krabben
ExSite PCR-kit from stratagene Ludwig Krabben
ExSite PCR-kit from stratagene Ludwig Krabben
Northern Hybridization Thomas Chuang
transform bacteria with linear DNA? Stock up and save. Limit one.
- transform bacteria with linear DNA? Rafael Maldonado
- transform bacteria with linear DNA? Brad Nicholson
- transform bacteria with linear DNA? Paul N Hengen
E-box binding bHLH protein antibody search William D. Woolfolk
BioRad Prep-a-gene Kit --recommendation Rich Kernan
- BioRad Prep-a-gene Kit --recommendation Andreas Enger
spectrophotometer purchase William H. Boylston
DNA synthesizer for sale Peter J. Myler
PCR problem Heavy E
PCR with Poly A primer jon boyson
ExSite PCR-kit from Stratagene Ludwig Krabben
looking for phage display vector Alex Chang
Experience with ABI Prism 310? J.Carnwath
CAT assays and transfection problems EILEEN
Alkaline-phosphetase extraction in E. coli jveitch at osprey.smcm.edu
detecting UV induced DNA damage Deepa Subramanian
manifold pipet "PBS::NELSONJ" at PBS.DFO.CA
Oligo probes and Northerns Garry P. Larson
library screening question Peter Thoren
PCR specificity? Peter
- PCR specificity? Beth Wapelhorst
Help: where to get fluorescin labelled dUTP? qyuan at gnv.ifas.ufl.edu
- Help: where to get fluorescin labelled dUTP? Jennifer Lyon
Mol Bio Software Frank P. Johnston
looking for pUC7 theveno at cc.umanitoba.ca
cytokines/in-situ-hyb.probes/in-situ-pcr georg lauer
Oligonucleotide staining Chia Jin Ngee
Manifold pipette (Dave Johnston)
(no subject) Oliver Politz
thermosequenase Oliver Politz
thermosequenase Oliver Politz
35SMet, 35SCys or 35SCys/Met ? Sandrine Imbeaud
- 35SMet, 35SCys or 35SCys/Met ? fdaniels at biosci.cbs.umn.edu
thermosequenase Sandrine Imbeaud
which electroporator ? Burkhard Hassel
- which electroporator ? Dima Klenchin
Database for phosphorylation sites? Graham Atherton
Lafmid BA - what is it? Sasha Kraev
Pre-made microsatellite oligos source? Robert
P1 phage/ plasmid query Stephen Perry
Re-use Easyprep wells COLINE at rulsfb.LeidenUniv.nl
Help needed on tissue printing pzhang
Rf: help with poly(A)+ mRNA purification LOGAND
(no subject) Alan Neely
Wanted: Human Small Intestinal cDNA library Srinivas K. Janardan, M.D.
Pre-made oligos for microsats-Source? Robert Roxby
Help: Can't see biotin:riboprobes in Agarose gels James Drummond
Custom coloning service Rinkei Ko
- Custom coloning service Onan
half life of Dynal magnetic beads? Eric Kofoid
Testosterone metabolite quantitation? Marko Kuittinen
Can I increase RT stringency? Darby Crash?
MS Conference Announcement irc at III.NET
which electroporator ? Sasha Kraev
- which electroporator ? Dima Klenchin
  - which electroporator ? steven morse
paper towels for blotting Kent Nastiuk, Ph.D.
Steroid Hormone Regulated Genes? ROBERT HARRISON
Help !- tRNA cont. of DNA prep. joao8528
Custom PCR services Rinkei Ko
- Custom PCR services Pete Muriana
Ni-NTA column and internal poly-His stretch. daniel durocher
- Ni-NTA column and internal poly-His stretch. Orhan
microsatellite resolution Peter Marko
Seeking spleen lambda expression library M Birkeland
- Seeking spleen lambda expression library mtc
test Bill Burnett
Commercial sequencing ops... Bill Burnett
- Commercial sequencing ops... martin LEACH
GFP Amadou Ba
- GFP Amadou Ba
mac prgram for creating silent mutations Kevin Wilson
- mac prgram for creating silent mutations gc
Beta-gal Non lethal Assays??? Graham Dellaire
Clontech "Matchmaker" vectors bicfo at flinders.edu.au
(no subject) doug davis
Methyl mercury treatment Jim Hutchins
- Methyl mercury treatment Kent Nastiuk, Ph.D.
mink lung epithelial cell line Vijayalaxmy Vijayan
Commercial source for deoxy-Inosine CED phosphoramidite John Marcus
Gel Smiling Andy Kravetz
- Gel Smiling Sarah Tamara Braun
- Gel Smiling Sui Huang
(no subject) rey garcia
actin as internal standard in RT-PCR rey garcia
transform bacteria with linear DNA? Duncan Clark
Yeast plasmid mini-preps Simon Dawson
database search sophia
looking for plasmids Patrick Van Dijck
Spectrophotometer John Webster
- Spectrophotometer Bill Burnett
Source for 9E10 Ab? Troels Wind
Help!!!Gel box cause for RNA degradation??? cad596 at lulu.acns.nwu.edu
- Help!!!Gel box cause for RNA degradation??? Margon Vandongen
looking for plasmids Patrick Van Dijck
Trimming 5' protrusive ends? Cocea
Can't find the gene! Hilde Nilsen
- Can't find the gene! jfh
Removal of glycogen from DNA J Ross
Commercial source of cre protein John Dixon
(no subject) David Ley
competent cells PRETORIUS
35SMet, 35SCys or 35SCys/Met ? fdaniels at BIOSCI.CBS.UMN.EDU
microtiter plates for PCR Christopher T. Cole
PCR Problems -- Help? James Chen
- PCR Problems -- Help? Rafael Maldonado
DNA sequence analysis via the net NAME
MutS, L, H protein source Parke K. Flick
Yeast plasmid mini-preps Gotz von Bulow
PCR Problems -- Help? Frederick Garbrecht
DNA synthesizer Peter J. Myler
DNA column purifications Michael Gorry
- DNA column purifications Duncan Clark
Peripheral Protien Purification Conrad R. Fjetland
Help on Transilluminators Javier Quintero Vazquez
- Help on Transilluminators Ned Mantei
Methyl mercury treatment Bradley Turner
Methyl mercury treatment Bradley Turner
MUltiple sequence Alignment Software Jean-Manuel Henry
Cheap ABI Dye Terminator Purification Steve Schramm
QIAGEN on the web? Not! (Yet!) QIAGEN
Commercial source of cre protein RVDESHP0 at PWINET.UPJ.COM
- Commercial source of cre protein John Dixon
Home-Made TA cloning Lauryl Maiya J Nutter
- Home-Made TA cloning Simon Dawson
ethidium bromide alternative? awam at hkucc.hku.hk
Gel Smiling Duncan Clark
Help!!!Gel box cause for RNA degradation??? Ned Mantei
flourescent stain for collagen? Mark Stowe
dephosphorylation buffer celer at brno.ics.muni.cz
Antibody to FMLP receptor? Mike Herron
ethidium bromide alternative? Sui Huang
Help!!!Gel box cause for RNA degradation??? Dan Baggott
Reproducible LA-PCR using BM Expander kit?? 8250
nucleic acid labeling and detection aarenas at interserv.com
Proliferation assay needed Claude SALIOU

Last message date: Sat Sep 30 22:13:44 EST 1995
Archived on: Sun Mar 6 20:44:58 EST 2005

Messages sorted by: [ subject ] [ author ] [ date ] Index

This archive was generated by Pipermail 0.09 (Mailman edition).