faint PCR bands

rlee rlee at INTERLINK.MDYN.COM
Mon Apr 1 19:54:32 EST 1996


     Dear Netters,
     
     I am trying to screen transgenic mice by PCR.  I have primers for 
     different exons of the gene and use them to check the existence of 
     each exon.   The products for some PCR reactions come up faint.  I 
     checked for primer dimers and didn't find that to be a problem; I also 
     used aerosol tips to eliminated contamination of the DNA's during 
     preparation.  What is a possible explanation for this phenomenon?
     
     Thank you in advance.
     
     Rozanda




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