proteinase pre-treatment

Chi-Kuang Wen cwen at aux.btny.purdue.edu
Mon Apr 1 18:00:42 EST 1996


Dear Netters:
   For the preparation of proteinase K, it is mentioned that proteinase K is 
not required for pre-treatment, but a pre-treatment is required for 
preparation of pronase to remove any DNase or RNase activity.

  Is there anyone can tell me why the pre-treatment is required for pronase 
but not for proteinase K?

    Someone said to me that the DNase and RNase are degraded immediately right 
after the addition of proteinase K.  Is it true?  Can someone show me citation 
related to this thought?

thanks,

Chi-Kuang

Chi-Kuang Wen
Botany & Plant Pathology
Purdue University
West Lafayette, IN 47907
USA

fax(work): (317)494-5896
phone(work):(317)494-0661



More information about the Methods mailing list