Cutting Qiagen miniprebs??

Michael Lassner mikel51 at pa.mother.com
Mon Apr 1 00:41:12 EST 1996


I've never had problems with digestion, but try this:
1) Spin down the bacterial lysate for an extra 5 minutes.
2) Use the optional PB wash.
3) Give the columns a second wash with PE buffer.
4)  Make extra sure to get the columns dry after the PE washes.  
Spin the columns for a minimum of 1 minute, and 2-3 minutes if you 
are not in a big hurry.Bjoern Voldborg wrote:
> 
> I am having serious problems cutting plasmids (pBlueskript) prepared with the Qiagen minipreb kit



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