Cutting Qiagen miniprebs??
mikel51 at pa.mother.com
Mon Apr 1 00:41:12 EST 1996
I've never had problems with digestion, but try this:
1) Spin down the bacterial lysate for an extra 5 minutes.
2) Use the optional PB wash.
3) Give the columns a second wash with PE buffer.
4) Make extra sure to get the columns dry after the PE washes.
Spin the columns for a minimum of 1 minute, and 2-3 minutes if you
are not in a big hurry.Bjoern Voldborg wrote:
> I am having serious problems cutting plasmids (pBlueskript) prepared with the Qiagen minipreb kit
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