plasmid cDNA library amplification

Maureen_L_Ho Maureen_L_Ho at sbphrd.com
Mon Apr 1 12:47:15 EST 1996


BRL has a protocol for a semi-solid agarose amplification that you can 
get from their web site.  Specifically it's title is GeneTrapper Protocol 
for Semi-solid Amplification of Plasmid cDNA Libraries.  One note, it 
says to use FMC's SeaPrep agarose, that is an absolute must.  I tried it 
with another prep grade agarose, and it was a mess. Otherwise this works 
well with a minimum of fuss, (no scraping cells).  Good luck!

Maureen Ho
-- 
The opinions expressed in this communication are my own,
  and do not necessarily reflect those of my employer.





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