plasmid cDNA library amplification
Maureen_L_Ho at sbphrd.com
Mon Apr 1 12:47:15 EST 1996
BRL has a protocol for a semi-solid agarose amplification that you can
get from their web site. Specifically it's title is GeneTrapper Protocol
for Semi-solid Amplification of Plasmid cDNA Libraries. One note, it
says to use FMC's SeaPrep agarose, that is an absolute must. I tried it
with another prep grade agarose, and it was a mess. Otherwise this works
well with a minimum of fuss, (no scraping cells). Good luck!
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and do not necessarily reflect those of my employer.
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