Getting cells off plates

S.K.Green S.K.Green at shef.ac.uk
Mon Apr 1 06:20:24 EST 1996


Pearse Ward wrote:
> 
> I'm having a problem getting COS-7 cells to dissociate from plastic
> tissue culture plates............I'd be interested in other people's favourite recipies for non-enzymatic dissociation of cells.
> 
> Many thanks,
> 
> Pearse

Dear Pearse,  We use small glass beads to shake off our cells. Works a 
treat!  Just autoclave some small (3mm) beads in tubes, add to your flask 
of cells, shake for a few seconds and pipette off cells.

Good luck,

Sue Green.



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