Getting cells off plates
S.K.Green at shef.ac.uk
Mon Apr 1 06:20:24 EST 1996
Pearse Ward wrote:
> I'm having a problem getting COS-7 cells to dissociate from plastic
> tissue culture plates............I'd be interested in other people's favourite recipies for non-enzymatic dissociation of cells.
> Many thanks,
Dear Pearse, We use small glass beads to shake off our cells. Works a
treat! Just autoclave some small (3mm) beads in tubes, add to your flask
of cells, shake for a few seconds and pipette off cells.
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