Subcloning sal1 insert into pBluescript

KHUANG at CHEMVX.TAMU.EDU KHUANG at CHEMVX.TAMU.EDU
Tue Apr 2 15:13:06 EST 1996


Hi, Netters,
I am trying to subclone sal1 insert from EMBL3 phage into pBluescript. I 
transformed the ligation mixture into TB1 by electroporation, many white 
clonies are visable, but when I did 15 minperps, no one has insert there.
Do you think sal1 is a difficult enzyme or TB1 is not suitable for pBluescript?
I purified the insert by Wizard PCR Kit, the ligase was from NEB. Thanks 

Ke-xue Huang
Department of Chemistry\
Texas A&M University



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