Ligation

john brennand john.brennand at gbapr.zeneca.com
Wed Apr 3 04:09:13 EST 1996


Adam

Yep the top strand isn't doing much to help the reaction in the second 
situation - but so what.

Single stranded DNA ligates just fine - as in SLIC (Single-stranded 
Ligation of cDNAs)

I don't really understand what problem you are trying to resolve

John





More information about the Methods mailing list