2D gels of basic proteins

Dr. Peter Friedhoff Friedhoff at MPASMB.DESY.DE
Wed Apr 3 10:29:41 EST 1996


Dear netters,

has anyone experience using immobilized pH-gradient 
gels 2D-gel electrophoresis of basic protein 
(e.g. pI around 10) ?
Furtermore I would like to know, how Immobiline II 
(Pharmacia) will work with the Acryl-amido-buffer 
(FLUKA) and how they perform in the basic pH region ?

Thanks for any help.
Peter




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