Correction factor for spectrophotometrical quantitation of DNA

john brennand john.brennand at gbapr.zeneca.com
Wed Apr 3 09:02:43 EST 1996


Cecilia

Standard DNA prep. protocols for animal cells/tissues should always 
include steps to eliminate protein, eg, via proteinaseK, 
Phenol/chloroform extraction, and ethanol precipitation of the DNA - so 
there should be very little there.  If there is in your samples, simply 
repeat this extraction.

Are you sure the overestimation you report isn't due to contaminating, 
RNAs, oligonucleotides or nucleotides - these are difficult to see on a 
gel but contribute significantly to A260.

This is the more likely "contaminant" from properly prepared DNA.

John





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