Correction factor for spectrophotometrical quantitation of DNA
john.brennand at gbapr.zeneca.com
Wed Apr 3 09:02:43 EST 1996
Standard DNA prep. protocols for animal cells/tissues should always
include steps to eliminate protein, eg, via proteinaseK,
Phenol/chloroform extraction, and ethanol precipitation of the DNA - so
there should be very little there. If there is in your samples, simply
repeat this extraction.
Are you sure the overestimation you report isn't due to contaminating,
RNAs, oligonucleotides or nucleotides - these are difficult to see on a
gel but contribute significantly to A260.
This is the more likely "contaminant" from properly prepared DNA.
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