Correction factor for spectrophotometrical quantitation of DNA

Cecilia Díaz Pohl cecilia.diaz-pohl at astra.se.astra.com
Wed Apr 3 07:27:04 EST 1996


I have noticed that quantitation of DNA based on absorbance measurements at 260 nm 
can give an overestimation of the amount of DNA when the samples are contaminated 
with proteins. This is often the case when the DNA has been extracted from animal 
tissue.
Does anybody know about some way of correcting for this?
 
Cecilia Díaz Pohl




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