Cloning up to 12kb into a eukaryotic expression vector, needing RNA transcription only.

Andrew Brooks andrew.brooks at
Thu Apr 4 13:05:36 EST 1996


I am looking for a eukaryotic expression vector (G418/neomycin 
selectable) into which I would like to clone up to 12Kb. I don't need 
translation. I only want constitutive transcription (inducible OK too) of 
the cloned fragment. The promotor should have a broad host cell range.

Can anyone offer some advice ?

Best Regards,


-------------------------------------------------------------------------Andrew Brooks                                          fax: 61-77-791 526
Dept of Biomedical and Tropical Veterinary Sciences  phone: 61-77-81 4518 
James Cook University of North Queensland
Townsville, Q, 4811

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