cloning up to 12Kb into a eukaryotic expression vector, needing RNA transcription only

Andrew Brooks andrew.brooks at jcu.edu.au
Thu Apr 4 12:50:48 EST 1996


Hi,

I am looking for a eukaryotic expression vector (G418/neomycin 
selectable) into which I would like to clone up to 12Kb. I don't need 
translation. I only want constitutive transcription (inducible OK too) of 
the cloned fragment. The promotor should have a broad host cell range.

Can anyone offer some advice ?



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