EtBr staining acrylamide gels

williams at bscr.uga.edu williams at bscr.uga.edu
Wed Apr 3 18:31:32 EST 1996


In article <Dp90Eo.AEC at midway.uchicago.edu>, dguttman at pondside.uchicago.edu (David S. Guttman) writes:
>Does anyone know if it is possible to incorporate EtBr dye into a
>polyacrylamide gel while casting so that the DNA stains is stained as
>it is run?
>
>Thanks in advance.
>
>Dave Guttman
>-----------------------------
>David S. Guttman
>Department of Ecology and Evolution
>University of Chicago
>312 702-8927 ph
>312 702-9740 fax
>dguttman at pondside.uchicago.edu

I've run flatbed submarine polyacrylamide gels with 0.1 ug/mL of EtBr (mixed
into acrylamide before polymerization); DNA bands were stained quite 
adequately.  The point to remember is that ethidium migrates 
toward the negative pole in electrophoresis (opposite direction from DNA) so 
you have to include an equal concentration of ethidium in the buffer at the 
bottom of the gel.

Good luck!

M. Williams
CCRC
University of Georgia





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