EtBr staining acrylamide gels

williams at williams at
Wed Apr 3 18:31:32 EST 1996

In article <Dp90Eo.AEC at>, dguttman at (David S. Guttman) writes:
>Does anyone know if it is possible to incorporate EtBr dye into a
>polyacrylamide gel while casting so that the DNA stains is stained as
>it is run?
>Thanks in advance.
>Dave Guttman
>David S. Guttman
>Department of Ecology and Evolution
>University of Chicago
>312 702-8927 ph
>312 702-9740 fax
>dguttman at

I've run flatbed submarine polyacrylamide gels with 0.1 ug/mL of EtBr (mixed
into acrylamide before polymerization); DNA bands were stained quite 
adequately.  The point to remember is that ethidium migrates 
toward the negative pole in electrophoresis (opposite direction from DNA) so 
you have to include an equal concentration of ethidium in the buffer at the 
bottom of the gel.

Good luck!

M. Williams
University of Georgia

More information about the Methods mailing list