Best inducible mammalian expression vector

Lisa Chakrabarti chakra at pasteur.fr
Thu Apr 4 04:54:03 EST 1996


Hi,

I plan to express a somewhat toxic protein from simian immunodeficiency
virus in a lymphoid cell line. Which inducible expression system will
work best ?

- the tetracycline-controlled vectors described by Gossen and Bujard
- vectors with metallothioneine promoter + heavy metals
- vectors with MMTV LTR + glucocorticoids
- the Lacswitch system from Stratagene with LacI-mediated repression
- any other system ?

Any info is welcome.
Many thanks.

Lisa



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