Searching for a CAT antibody !
milstone at rascal.med.harvard.edu
Thu Apr 4 09:37:38 EST 1996
As of several years ago, the only available anti-CAT antibodies were
problematic, at best. Positive staining required very high levels of CAT
protein, especially compared to the low levels detectable by the
enzymatic assay for CAT. There was, and may still be, a commercial
source for these Abs. My impression is that, unless you are absolutely
committed to visualizing CAT as a reporter gene product (e.g., you must
analyze irreplaceable samples from previous experiments performed with
CAT), that you will be much better off switching to another reporter,
such as luciferase (Abs are available) or beta-galactosidase (Abs are
available & histocheimstry is easy), which is more easily localized.
This is true even if you must repeat a some experiments.
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