[Na] and Tm of oligos...

Bill Burnett bbur at wpo.nerc.ac.uk
Wed Apr 3 11:04:03 EST 1996

Possibly a dumb question but...  Why do melting point determination formulas 
include sodium ion concentration... and how, for my sodiumless PCR's and cycle 
sequencing reactions, do I try and predict Tm for my primers?  (There's no Na 
in my templates, and I don't add any Na2EDTA or anything else with Na in it...)



Bill Burnett - bbur at wpo.nerc.ac.uk       |  Not so much the slow
Scottish Association for Marine Science  |  lane as the hard shoulder...
P.O. Box 3, Oban, Argyll PA34 4AD, UK    |

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