GST-Fusion protein proteolysis

Brian J Murphy bmurphy at U.WASHINGTON.EDU
Fri Apr 5 13:40:00 EST 1996

Does anyone have any references or understanding of the complement of 
proteases in E. coli.?  The major bacterial proteases cleave proteins 
after double Arginines or Lysines.  What protease inhibitors block these 
proteases?  Are the regular complement of protease inhibitors used in 
protein purification effective against coli proteases?

Look forward to any help.

Brian Murphy 
Dept. Pharmacology 
University of Washington 

More information about the Methods mailing list