Help with Electroporation!!!

tera tjstbc at sd.cts.com
Tue Apr 9 15:08:55 EST 1996


Peter,

We use the same electroporator, however, the 0.2 cm cuvettes work best 
for eukaryotic cells - we use 0.1cm cuvettes and 1.88 Kv.  Also, best 
results have been achieved by ethanol ppt. ligation prior to 
electroporation. Hope this helps. Regards. Barry/Tera.





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