Linear acrylamide (was --Re: glycogen as a carrier)

AShaw5 ashaw5 at aol.com
Thu Apr 11 00:29:14 EST 1996


Abolutely right.  It always surprises me to see these very old and
classical procedures listed.  We used to make acrylamide carrier by
polymerizing some acyramide (3-5%).  We then take a needle and homogenize
the polymerized acrylamide.  It is then spun down to get rid of the big
insoluble fragments.  The sup should contain sheared linear polymerized
acrylamide which precipitates in ethanol.

Andrey Shaw



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