How to crack yeast for PCR ??

Mark Prescott Mark.Prescott at med.monash.edu.au
Thu Apr 11 18:32:11 EST 1996


news:hindges-1104961158050001 at 130.60.120.11
hindges at vetbio.unizh.ch (Robert Hindges) wrote:
>In article <liss.15.316BD4A9 at alf1.ngate.uni-regensburg.de>,
>liss at alf1.ngate.uni-regensburg.de (Michael Liss t4310) wrote:
>
>cut
>> 
>> Let me come to my question:
>> Is this DNA-isolation really necessary or is there an easier way to
>> perform a reliable PCR. (Just like with E. coli where you just take a 
>> little colony and add the whole cells to your reaction.)
>> If anybody has experience with this or can contribute any other 
>> suggestions on how to run the PCR etc. please let me know...
>> 
>> Thanx in advance.
>> 
>> Mike
>
>Mike
>
>I used the method from G.M.Sathe et al. described in NAR (19) 17:4775
>(1991) for detecting insert sizes in my plasmids. But even with that
>method I only found about 1/5 of all samples working. So I went back to
>extract plasmids before PCR to have reliable results. I would be very
>interestted too if someone else has a good protocol for it.
>
>robert

Mike,
Try the protocol in Nucleic Acids Research vol 23, 4924-4925 (1995).  The 
method is simple, rapid and extremely reliable in our hands.
Good Luck.
Mark




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