DEAE-transfection of EL-4 cells

Paul D. Boyer, Ph.D. boyer at
Thu Apr 11 14:59:49 EST 1996

hyho at GATE.SINICA.EDU.TW (Hung-yiu Ho) wrote:

>Dear netter,
>  What is the optimal condition (i.e. DEAE-dextran concentration and 
>duration of DMSO shock) for EL-4 cells? I have tried very hard to test 
>it. Please help me!
The best source I know is Tom Novak, who worked very hard as a grad.
student at Caltech to optimize the conditions for these assays.  He's
now at Wyeth-Ayerst, and his e-mail address is
novakt at

As far as I know, he's the best!  He published this work in Mol. Cell.
Biol.  Ask him for the reference.

Paul D. Boyer, Ph.D.         University of Wisconsin-Parkside            
Assistant Professor                   900 Wood Road, Box 2000
Department of Biological Sciences     Kenosha, WI  53141-2000
Biomedical Research Institute        e-mail: boyer at

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