Gene synthesis using oligos

Dr. Duncan Clark duncan at
Thu Apr 11 08:10:22 EST 1996

Hi Folks,

I need to synthesise the first 130bp of a gene. Rather than try to using
two v.large oligos I thought of using four smaller overlapping ones and
then PCR'ing using the two outers or using klenow to fill in the gaps.
My question is how many bases should I overlap on each oligo? Is a
homolgy of ten bases at each end of an oligo enough?

      start --------------->         --------------->
                         <--------------          <-------------- end

Many thanks


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

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