help with ligation

Denise M. Gibo dgibo at prs.hmc.psu.edu
Thu Apr 11 13:51:44 EST 1996


Yes, Edta can inhibit your T4 ligase.(according to Gibco/BRL)  I would
recommend dissolving your DNA in water and then trying your ligations
with a variety of ratios including a "vector alone" control.  You may
also try a longer ligation at a lower temp. like 4-14 C overnight.



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