Ligation

Chris Boyd chrisb at hgu.mrc.ac.uk
Thu Apr 11 07:01:37 EST 1996


john brennand (john.brennand at gbapr.zeneca.com) wrote:

: Yep the top strand isn't doing much to help the reaction in the second 
: situation - but so what.

: Single stranded DNA ligates just fine - as in SLIC (Single-stranded 
: Ligation of cDNAs)

Nice try, John, but no banana.  ssDNA ligation works with T4 *RNA*
ligase, not with T4 *DNA* ligase: see, eg, S. Kaluz et al.,
Biotechniques (1995), 19:182. Unfortunately, one has to infer that T4
DNA ligase works efficiently only on dsDNA-dsDNA joining.

Best wishes,
--
Chris Boyd                       | from, | MRC Human Genetics Unit
chrisb at hgu.mrc.ac.uk             |  not  |  Western General Hospital
http://www.hgu.mrc.ac.uk/~chrisb |   for |   Edinburgh EH4 2XU, SCOTLAND




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