Gene synthesis using oligos

Rafael Maldonado rafael at corona.med.utah.edu
Fri Apr 12 16:03:20 EST 1996


On Thu, 11 Apr 1996, Dr. Duncan Clark wrote:
> 
> 
>       start --------------->         --------------->
>                          <--------------          <-------------- end
> 


I forgot tell something about that. 10 bases is enough, but the 
temperature of incubation of the klenow reaction should be lower that the 
melting posint of the oberlapping sequence. An overnight incubation at 
25 C should be OK. 


Rafa

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