NdeI: cutting problems?? anyone else?? seems silly! (fwd)

[PRETORIUS]

In article <dmorris-1503961110250001 at thermo.sbs.auckland.ac.nz>, dmorris at sbsnov1.auckland.ac.nz (daniel morris) says:
>
>Ditto.  Use lots of enzyme, overnight incubations preferably, add more for
>good luck every now and then, use CLEAN DNA, and allow at least six
>base-pairs of extra sequence when designing primers containing terminal
>NdeI sites.
>
>Its a bastard of an enzyme.



It also helps if one increases the digestion volume.

Asha