cloning amplicons with inosine

vilimf01 at mcrcr6.med.nyu.edu vilimf01 at mcrcr6.med.nyu.edu
Fri Apr 12 19:23:43 EST 1996


When I did it (T/A cloning) the inosines were converted to C's in the
plasmids.


In article <4kmch0$9hk at news.cis.okstate.edu>, demed at vms.ucc.okstate.edu. (David Demezas) writes:
> I have a set of primers with several inosines in each primer.  I was
> wondering if the amplified products from these primers can be cloned? 
> Does anyone know what nucleotides will be used to complement inosine
> when the 
> cloned fragment is replicated?  Thanks in advance for your help.  



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