DNase cuts

luis brieba lgbrieba at academ01.mty.itesm.mx
Sun Apr 14 20:32:46 EST 1996


	Hi!!!!!!
	
	We are traing to do DNase cuts of a gen of 2.4 kD. We hope to obtain 
products between 30 to 250 base pairs and use them in a posterior 
reaction.
 Does anybody know how to improve the kinetics of DNAse (bovine 
pranceatic) for a specifc range of cut? or have experience using the 
enzyme supply by Amhersam?

 The first time that we do that, we can not visuale the cuts in agarose 
2%.

																								Thanks,


																																								Luis





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