Gibco Stbl2 cells: deletion curse!

walter wlech at
Mon Apr 15 18:53:45 EST 1996

We have a 12kb plasmid that continually gets deleted ~75% of the time. 
We'll pick a fresh colony (<2 days old) and innoculate 25, 50 or 100ml 
of LB or TB. Growth is at 30 or 32 deg C for 15hrs. Has anybody had 
similar problems with these cells, or any suggestions? 

Walter Lech

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