Radiolabelling proteins in Coli
john.brennand at gbapr.zeneca.com
Mon Apr 15 09:49:23 EST 1996
We are having trouble visualising a recombinant protein on coomassie
gels. So, has anyone got a good "money back" protocol for radiolabelling
newly synthesised proteins in E Coli following induction (iptg) of
expression from a plasmid ?
What I need to know are things like:
Growth state of the cells, media composition, which radiolabel works
best, how much label to use, time of incubation, etc.
I guess I could do experiments to work all of this out but I just want a
quick answer to whether our construct is making any recombinant protein
and if so, is it being degraded intracellularly
Thanks for any help
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