Knock-out of bacterial gene

[Jose M. Bautista]

Does anyone have any good protocols/ideas for knocking-out a bacterial 
gene (E.coli) of known sequence?


Thanks a lot

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.       Jose M. Bautista                                        .
.       Department of Haematology                               .
.       Royal Postgraduate Medical School                       .
.       Hammersmith Hospital, Du Cane Road, London W12 0NN,UK   .
.       Tel + 44 181 743 2030 ext. 2162                         .
.       Fax + 44 181 742 9335                                   .
.       e-mail boxes:   bauchem at eucmax.sim.ucm.es               .
.                       jbautist at hgmp.mrc.ac.uk                 .
.                       jmbautista at samba.cnb.uam.es             .
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